Full Length Research Paper
Abstract
The aim of this study was to obtain a freeze-dried extract from Issopus officinalis with an important antioxidant and antimicrobial effect. The extract was obtained in a fluidized bed solid extractor and the optimal values of phenols, flavonoids and the highest reduction power were determined when using 75% ethanol as solvent, the antioxidant activity ranging between 90 to 95%. In case of the freeze-dried product, the maximum antioxidant activity was registered when using 0.5 mg/ml powder and the results were optimal as well in case of determining flavonoids, phenols and the reducing power. The antimicrobial effect of the extract and of the freeze-dried product were quantified by using the Colony Quant software on the strains Escherichia coli CBAB 2, Bacillus cereus CMGB 215, Listeria innocuaCMGB 218. An inhibition area of maximum 2 cm resulted when using 75% ethanol as solvent. The optimal inhibitory effect of the freeze-dried product of around 1.7 cm was determined by a 0.5 mg/ml concentration.
Key words: Extraction, ethanol, 1.1 – diphenyl – 2 – Picrylhydrazyl, bacteria, colony quant.
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