Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3830

Full Length Research Paper

The obtaining of an antioxidant product based on Issopus officinalis freeze: Dried extract

Vamanu Emanuel1,2, Vamanu Adrian1,2, Niţă Sultana3 and Colceriu Svetlana3
1Applied Biochemistry and Biotechnology Center – Biotehnol, Bd. Mărăşti no. 59, sector 1, Bucharest, Romania. 2Faculty of Biotechnology, University of Agronomic Sciences and Veterinary Medicine, Bd. Mărăşti no. 59, Sector 1, Bucharest, Romania. 3National Institute of Chemical-Pharmaceutical Research-Development – ICCF Bucharest, Vitan Road no. 112, Sector 3, Bucharest, Romania.
Email: [email protected]

  •  Accepted: 03 March 2011
  •  Published: 18 May 2011

Abstract

The aim of this study was to obtain a freeze-dried extract from Issopus officinalis with an important antioxidant and antimicrobial effect. The extract was obtained in a fluidized bed solid extractor and the optimal values of phenols, flavonoids and the highest reduction power were determined when using 75% ethanol as solvent, the antioxidant activity ranging between 90 to 95%. In case of the freeze-dried product, the maximum antioxidant activity was registered when using 0.5 mg/ml powder and the results were optimal as well in case of determining flavonoids, phenols and the reducing power. The antimicrobial effect of the extract and of the freeze-dried product were quantified by using the Colony Quant software on the strains Escherichia coli CBAB 2, Bacillus cereus CMGB 215, Listeria innocuaCMGB 218. An inhibition area of maximum 2 cm resulted when using 75% ethanol as solvent. The optimal inhibitory effect of the freeze-dried product of around 1.7 cm was determined by a 0.5 mg/ml concentration.

 

Key words: Extraction, ethanol, 1.1 – diphenyl – 2 – Picrylhydrazyl, bacteriacolony quant.