Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3818

Full Length Research Paper

The protective effect of Chrysanthemum fantanesii extract, vitamin E and C on sodium valproate-induced embryotoxicity in pregnant mice

Amrani Amel1, Zama Djamila1*, Boubekri Nassima1, Benaissa Ouahiba1, Meraihi Zahia2 Benayache Fadila3, Benayache Samir1 and Bettuzzi Saverio4
1Laboratoire de Valorisation des Ressources Naturelles et Synthèses de Substances Biologiquement Actives, Faculté des Science Exactes, Université Mentouri, Constantine, Algérie. 2Laboratoire de Génie Microbiologique et Applications, Département des Sciences de la Vie et de la Nature, Faculté des Sciences Université Mentouri, 25000 Constantine, Algeria. 3Laboratoire de Phytochimie et Analyses Physico-Chimiques et Biologiques, Faculté des Science Exactes, Université Mentouri, Constantine, Algérie. 4Dipartimento di Medicina Sperimentale, Sezione di Biochimica, Biochimica Clinica e Biochimica dell'Esercizio Fisico, Plesso Biotecnologico Integrato, Università degli Studi di Parma, via Volturno 39, 43100 Parma, Italy.
Email: [email protected]

  •  Accepted: 30 November 2011
  •  Published: 23 May 2012


Valproate (VPA)  has been shown to induce neural tube defect in human and mice. In this study, we investigated the ability of butanolic extract from leaves of Chrysanthemum fantanesii, vitamin E and C to modulate VPA-induced toxicity and oxidation damage in maternal and fetal tissues in mice. Plant extract, VPA and vitamins were administered orally to pregnant mice from the 6 to 17th day of gestation. Animals received plant extract (200 mg/kg per day), vitamin E (100 mg/kg per day) and vitamin C (8.3 mg/kg per day) with an oral administration of VPA (400 mg /kg per day) under the same conditions. On day 18 of gestation pregnant mice were sacrificed, fetuses, placenta and maternal tissues were removed, homogenized and used for the determination of lipid peroxidation rates (LPO) using thiobarbituric acid reactive substance (TBARS) method. Embryotoxicity wasassessed by counting the number of implants, live and dead fetuses, and resumptions. Thefetuses were observed for malformation including neural tube defect (Exencephaly), open eyes and skeletal malformation. The results show clearly that there is a positive correlation between the increase in LPO and congenital malformation. Plant extract, vitamin E and C caused partial decrease of embryo toxicity and congenital malformation induced by VPA in mice.


Key words: Valproic acid, congenital malformation, embryotoxicity, oxidative stress, lipid peroxidation, Chrysanthemum fantanesii, vitamin E and C.