Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3750

Full Length Research Paper

Pachyrhizus spp. seed extracts control the Sclerotium rolfsii Sacc. mycelial growth

Rodrigo Gonçalves de Lima
  • Rodrigo Gonçalves de Lima
  • Biotechnology School, Federal University of Amazonas, Amazonas State, Brazil.
  • Google Scholar
César Augusto Ticona-Benavente
  • César Augusto Ticona-Benavente
  • Coordination of Society, Environment, and Health, National Institute for Amazonian Research, Amazonas State, Manaus, Brazil.
  • Google Scholar
Danilo Fernandes da Silva Filho
  • Danilo Fernandes da Silva Filho
  • Coordination of Society, Environment, and Health, National Institute for Amazonian Research, Amazonas State, Manaus, Brazil.
  • Google Scholar
Leandro Sousa e Silva
  • Leandro Sousa e Silva
  • Tropical-Humid Agriculture School, National Institute for Amazonian Research, Amazonas State, Manaus, Brazil.
  • Google Scholar
Rândrea Grazziella Verçosa Guimarães
  • Rândrea Grazziella Verçosa Guimarães
  • Tropical-Humid Agriculture School, National Institute for Amazonian Research, Amazonas State, Manaus, Brazil.
  • Google Scholar
Rogerio Eiji Hanada
  • Rogerio Eiji Hanada
  • Tropical-Humid Agriculture School, National Institute for Amazonian Research, Amazonas State, Manaus, Brazil.
  • Google Scholar


  •  Received: 16 January 2021
  •  Accepted: 20 May 2021
  •  Published: 31 July 2021

Abstract

The present paper aimed to characterize the bioactivity being exerted on Sclerotium rolfsii Sacc. in vitro by seed aqueous extracts from 64 yam bean (Pachyrhizus spp.) genotypes as well as their maceration times (0, 48 and 72 h). Three experiments were carried out following a completely randomized design with 64 treatments (potato-dextrose-agar PDA+extracts) with two controls (PDA with no extract and PDA+Cabrio® Top in the dose of 4 g/L) with four replicates with one Petri dish per experimental unit. The 64 treatments were adjusted in the dose of 0.1%. The mycelial growth diameter was assessed on the fifth day. Findings showed the diameters of macerating extracts to be 34.3 - 67.0, 23.5 - 57.8, and 37.5 - 63.8 mm at 0, 48, and 72 h, respectively. PDA+Cabrio® Top and PDA with no extract controls ranged from 0.05 to 0.50 mm and 73.2 to 80.7 mm, respectively. P14, P1, P15, and P46 genotypes extracts achieved the lowest mean diameters (47 - 48% shorter). P61 was the genotype bearing the largest mean diameter tantamount to a reduction of 21%. Therefore, every aqueous extract has shown to be active against S. rolfsii, which indicates that genotypes exerting higher toxicity should either be tested against other pathogens or used in the field macerating them for 48 h.

Key words: Jacatupé, yam beam, secondary metabolites, toxicity, natural fungicides.