Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Efficient propagation of an important medicinal plant Boesenbergia rotunda by shoot derived callus

Nor Azma Yusuf1,2*, M. Suffian M. Annuar1 and Norzulaani Khalid1
1Centre of Biotechnology for Research in Agriculture, Biotechnology and Bioproduct Research Cluster, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. 2Faculty of Plantation and Agrotechnology, Faculty of Applied Sciences, University Technology MARA (UiTM) 40450 Shah Alam, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 22 March 2011
  •  Published: 04 July 2011


A protocol for high frequency shoot organogenesis and plant establishment from shoot base derived callus was developed for Boesenbergia rotunda (L.) Mansf. Kulturpfl. The cultures produced callus in MS medium supplemented with various concentrations of dicamba and 2,4-D. Nevertheless, embryogenic callus only emerged on 2, 4-D augmented MS medium. In addition, the success of callus induction was achieved from meristem derived callus producing 100% of embryogenic callus. Multiple shoot induction was achieved from the surface of the callus after transferring onto MS medium supplemented with BAP or kinetin (shoot induction medium). The highest frequency of callus producing shoots (49.8%) was achieved on MS medium containing 2.0 mg/L BAP. Plantlets with well-developed shoots and roots on MS medium supplemented with 2 g/L activated charcoal were acclimatized and successfully grown in the greenhouse.


Key wordsBoesenbergia rotunda, plant cell cultures, medicinal plant, organogenesis, Zingiberaceae, micropropagation.