Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Enhanced production of mycelial biomass and extracellular polysaccharides in caterpillar-shaped medicinal mushroom Cordyceps sinensis CS001 by the addition of palmitic acid

Xiao-Ling Wang, Gao-Qiang Liu*, Chao-Yang Zhu, Guo-Ying Zhou and Si-Min Kuang
Hunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, P. R. China.
Email: [email protected]

  •  Accepted: 08 March 2011
  •  Published: 04 July 2011

Abstract

To screen stimulators for cell growth and polysaccharides production of caterpillar-shaped Chinese medicinal mushroom, Cordyceps sinensis, the fungus was inoculated into the submerged culture media with and without supplementation of several fatty acids and ether extracts of Chinese medicinal insects. The results showed that palmitic acid at 1.0 g/l lead to significant increases in both biomass and extracellular polysaccharides (EPS) concentration (C) from 8.26 ± 0.34 to 11.10 ± 0.39 g/l and 353.1 ± 11.5 to 431.2 ± 13.8 mg/l, respectively. In addition, ether extract of medicinal insect, Eupolyphaga sinensis at 1.5 g/l was also of advantage to biomass and EPS production. There were no new components in the EPS obtained by the addition of palmitic acid.

 

Key words: Cordyceps sinensis, medicinal mushroom, extracellular polysaccharides, palmitic acid, submerged culture.