Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Chemical composition, bio-herbicidal and antifungal activities of essential oils isolated from Tunisian common cypress (Cupressus sempervirens L.)

Amri Ismail1*, Hamrouni Lamia1, 2, Hanana Mohsen3, Gargouri Samia4 and Jamoussi Bassem5    
1Département de Biologie, Faculté des Sciences de Bizerte. Zarzouna, 7021 Bizerte, Tunisie. 2Laboratoire d’Ecologie Forestière, Institut National de Recherches en Génie Rural, Eaux et Forêts. BP 10, 2080 Ariana, Tunisie. 3Laboratoire de Physiologie Moléculaire des Plantes, Centre de Biotechnologie de Borj-Cédria. BP 901, 2050 Hammam-lif, Tunisie. 4Laboratoire de Protection des Végétaux, Institut National de la Recherche  Agronomique de Tunisie. Rue Hédi Karray, 2080 Ariana, Tunisie. 5Institut Supérieur d’Education  et de Formation Continue. Tunis, Tunisie.    
Email: [email protected]

  •  Accepted: 11 January 2013
  •  Published: 25 April 2013


The chemical composition of essential oils obtained by hydrodistillation from leaves, branches and female cones of Tunisian Cupressus sempervirens L. was determined by gas chromatography (GC) and CG-mass spectrometry (GC/MS) analysis, 52 compounds were identified; qualitative and quantitative differences between oils were observed. All oils were rich in monoterpene hydrocarbons, and the major constituents were α-pinene (27.5 to 35.8%), α-cedrol (7.7 to 19.3%), δ-3-carene (5.8 to 13.2%) and germacrene D (3.9 to 12.1%). Essential oils of C. sempervirens have shown a significant phytotoxic effect against the germination and seedling growth of four weeds: Sinapis arvensis L.Trifolium campestre Schreb (dicots), Lolium rigidum Gaud and Phalaris canariensis L. (monocots). Tested oils strongly inhibited the germination and seedling growth of all weeds, in a dose dependent manner. The in vitro antifungal activity of the essential oil samples of C. sempervirens were evaluated against 10 cultivated crop fungi, and all samples have shown a significant antifungal activity against all tested fungi and it can be suggested to have the potential to be used as a bio-herbicide and alternatives fungicide.


Key words: Cupressus sempervirens, essential oils, bio-herbicidal activity, antifungal potential, weeds, allelopathy.