Full Length Research Paper
The chemical composition of essential oils obtained by hydrodistillation from leaves, branches and female cones of Tunisian Cupressus sempervirens L. was determined by gas chromatography (GC) and CG-mass spectrometry (GC/MS) analysis, 52 compounds were identified; qualitative and quantitative differences between oils were observed. All oils were rich in monoterpene hydrocarbons, and the major constituents were α-pinene (27.5 to 35.8%), α-cedrol (7.7 to 19.3%), δ-3-carene (5.8 to 13.2%) and germacrene D (3.9 to 12.1%). Essential oils of C. sempervirens have shown a significant phytotoxic effect against the germination and seedling growth of four weeds: Sinapis arvensis L., Trifolium campestre Schreb (dicots), Lolium rigidum Gaud and Phalaris canariensis L. (monocots). Tested oils strongly inhibited the germination and seedling growth of all weeds, in a dose dependent manner. The in vitro antifungal activity of the essential oil samples of C. sempervirens were evaluated against 10 cultivated crop fungi, and all samples have shown a significant antifungal activity against all tested fungi and it can be suggested to have the potential to be used as a bio-herbicide and alternatives fungicide.
Key words: Cupressus sempervirens, essential oils, bio-herbicidal activity, antifungal potential, weeds, allelopathy.
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