Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3672

Full Length Research Paper

In vitro and in vivo evaluation of quinones from Auxemma oncocalyx Taub. on Leishmania braziliensis

Sayonara de Melo Viana1, Maria Augusta D. Ferreira2, Priscila Valera Guerra1, Glauce S. Barros Viana3 and Maria Jania Teixeira1*  
1Departamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade Federal do Ceará (UFC), Rua Alexandre Baraúna, 949, 60.430-160 Fortaleza, CE, Brazil. 2Departamento de Farmácia, Faculdade de Farmácia, Odontologia e Enfermagem, UFC, Rua Capitão Francisco Pedro 1210, 60430-370 Fortaleza, CE, Brazil. 3Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, UFC, Av. Cel. Nunes de Melo, 1127, 60430-270 Fortaleza, CE, Brazil.
Email: [email protected]

  •  Received: 25 June 2013
  •  Published: 03 February 2015

Abstract

The effect of a quinone fraction (QF) isolated from the heartwood of Auxemma oncocalyx Taub. was investigated in vitro and in vivo on Leishmania braziliensis. Although QF (1-10 μg/mL) showing marked in vitro anti-Leishmania activity (81 to 94%), the oral treatment with the compound did not protect hamsters against progressive L. braziliensis infection. When QF was administered intraperitoneally (20 mg/kg) the lesion size was reduced by 65%; however, it was not able to promote parasite eradication, as evidenced by the high number of parasites in draining lymph nodes. Quinones are highly redox active molecules and with their semiquinones radicals can lead to formation of reactive oxygen species (ROS). The generation of ROS could probably explain the in vitro leishmanicidal activity of the quinones, since promastigotes are susceptible to H2O2 lethal effect in vitro. In conclusion, although quinones seem to be effective against Leishmania parasites in vitro, they do not demonstrate a therapeutic effect in experimental leishmaniasis. In addition, it can be hypothesized that QF in vivo might possibly be converted into non-active metabolite(s) or be inactivated either by reduction or by interaction with serum proteins, losing its leishmanicidal activity.

 

Key words: Leishmania braziliensis, Auxemma oncocalyx, quinone, hamster, cutaneous leishmaniasis.