Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Development of a rich fraction in phenolic compounds with high antioxidant and antimicrobial activity in Amburana cearensis seeds extracts

Aline Aires Aguiar
  • Aline Aires Aguiar
  • Natural Products Research Laboratory, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte), Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
  • Google Scholar
Ilsamar Mendes Soares
  • Ilsamar Mendes Soares
  • Natural Products Research Laboratory, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte), Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
  • Google Scholar
Poliana Guerino Marson
  • Poliana Guerino Marson
  • Faculty of Medicine, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte), Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
  • Google Scholar
Ernane Gerre Pereira Bastos
  • Ernane Gerre Pereira Bastos
  • General and Applied Microbiology Laboratory, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte), Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
  • Google Scholar
Sérgio Donizeti Ascêncio
  • Sérgio Donizeti Ascêncio
  • Faculty of Medicine, Natural Products Research Laboratory, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte), Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
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Raimundo Wagner de Souza Aguiar
  • Raimundo Wagner de Souza Aguiar
  • Department of Biotechnology, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte) Gurupi, Federal University of Tocantins, Tocantins 77404-970, Brazil.
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  •  Received: 22 September 2017
  •  Accepted: 23 October 2017
  •  Published: 03 November 2017

Abstract

Amburana cearensis is a medicinal plant widely used in folk medicine. The purpose of this study was to identify the more appropriate extraction solvent for maximum antioxidant and antimicrobial effect. The extraction of A. cearensis seeds were carried out gradually to obtain the highest yields and constituents of the extracts using as solvent hexane, methanol, 80% alcohol and water in this sequence. Phytochemical screening showed phenolic compounds and thin layer chromatography (TLC) showed the flavonoid morin. Antioxidant activity was also evaluated by the method of scavenging the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH); rutin and ascorbic acid was used as standard; the hydro-alcoholic extract (EEA) showed an IC50 value of 17.95 μg/ml, similarly to the standards rutin and ascorbic acid, indicating high antioxidant action. The microdilution assay (MIC) showed antibacterial activity against the bacteria concentrations, values ≥0.25 mg/mL for EEA and aqueous (AEA) extracts against S. aureus, P. aeruginosa and S. flexneri. High performance liquid chromatography (HPLC) analysis of A. cearenses seed extracts revealed a high content of flavonoids and tannin compounds corroborating with TLC analysis. The extraction by exhaustion was very effective in exposing the bioactive principles of A. cearenses seeds showing their best compounds mainly in EEA extract.

 

Key words: Amburana cearensis, exhaustion extraction, seed, phytochemical profile, antioxidant, antimicrobial activity.