Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3704

Full Length Research Paper

Anti-inflammatory and antinociceptive activity of field-growth plants and tissue culture of Cleome spinosa (Jacq.) in mice

Norma Albarello1*, Claudia Simões-Gurgel1, Tatiana Carvalho de Castro1, Carlos Roberto M. Gayer2, Marsen Garcia Pinto Coelho2, Roberto Soares de Moura3 and Elisabeth Mansur4    
1Departamento de Biologia Vegetal - DBV/IBRAG. R. São Francisco Xavier, 524, PHLC, sala 509. Maracanã, RJ. Brasil. CEP: 20.550-013. Universidade do Estado do Rio de Janeiro (UERJ). 2Departamento de Bioquímica - DBq/IBRAG. Av. 28 de Setembro, 87 fundos. 4o. Andar. Vila Isabel, RJ. Brasil. CEP: 20.551-030. Universidade do Estado do Rio de Janeiro (UERJ). 3Departamento de Farmacologia e Psicobiologia - DFP/IBRAG. Av. 28 de Setembro, 87 fundos. 5o. Andar. Vila Isabel, RJ. Brasil. CEP: 20.551-030. Universidade do Estado do Rio de Janeiro (UERJ). 4Departamento de Biologia Celular - DBCel/IBRAG. R. São Francisco Xavier, 524, PHLC, sala 505. Maracanã, RJ. Brasil. CEP: 20.550-013. Universidade do Estado do Rio de Janeiro (UERJ).    
Email: [email protected]

  •  Accepted: 29 June 2012
  •  Published: 25 April 2013

Abstract

Methanol extracts (ME) of Cleome spinosa obtained from plants of natural growing areas (NP) and acclimatized plants (AP), as well as from the lipophilic mass containing beta-carotene (LMBC) produced from callus cultures were evaluated for their anti-inflammatory and antinociceptive potentials. These properties were studied using carrageenan-induced paw edema and acetic acid-induced writhing animal models. The ME from leaves of NP and AP 10 mg/kg, intraperitoneally (i.p.) caused a significant inhibition of carrageenan-induced paw edema. Antinociceptive activity was observed in extracts from leaves of AP, leaves and stems of NP and LMBC, at 50 mg/kg (i.p.), corresponding to half concentration of the standard drug dipyrone (100 mg/kg). The extracts did not present toxicity as determined by Artemia salina bioassay. Our results demonstrated that C. spinosa has anti-inflammatory and antinociceptive properties, confirming its popular use, and also served to extend the use of plant biotechnology tools for investigations on the pharmacological potential and phytochemical exploitation of this species.

 

Key words: Cleomaceae, analgesic effect, anti-inflammation, plant tissue culture, beta carotene.