Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3793

Full Length Research Paper

Topical application of Calendula officinalis (L.): Formulation and evaluation of hydrophilic cream with antioxidant activity

Jurga Bernatoniene1*, Ruta Masteikova2, Julija Davalgiene1, Rimantas Peciura1, Romualda Gauryliene3, Ruta Bernatoniene1, Daiva Majiene1, Robertas Lazauskas1, Genuvaite Civinskiene4, Saule Velziene1, Jan Muselik2 and Zuzana Chalupova2
1Department of Drug Technology and Pharmaceutical Management, Faculty of Pharmacy, Kaunas University of Medicine, Kaunas, Lithuania. 2Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic. 3Kaunas College, Faculty of Healthcare, Kaunas, Lithuania. 4Department of Physiology, Faculty of Pharmacy, Kaunas University of Medicine, Kaunas, Lithuania.
Email: [email protected]

  •  Accepted: 14 July 2010
  •  Published: 18 March 2011


The aim of the study was the formulation of suitable medicinal form for the topical application of Calendula officinalis L. Dry Calendula extract was used as an active compound. This extract was proved to be an effective scavenger of H2O2 radicals in in vitrostudies with the mitochondria of rat cardiac muscles. Several compositions of the cream base were evaluated and the hydrophilic cream containing complex emulsifier was chosen as the delivery system. Subsequently, Calendula extract was incorporated, and the concentration of extract which provided significant antioxidant effect (p < 0.05), has been determined. Antioxidant activity of the cream with Calendula extract was due to the content of carotenoids, polyphenols and flavonoids. Cream with the best properties (0.9% of Calendula extract) contained 0.73 ± 0.04 mg/100 g of total carotenoids expressed as β-carotene. This cream was then examined microscopically, and stability studies including evaluation of organoleptic properties, microbiologic quality, and determination of variations in total carotenoid content during the storage were made. Achieved results suggest that developed cream with Calendula extract poses the good-quality emulsion system warranting the stability of carotenoids, and thus the therapeutic, namely antioxidant activity of preparation.   


Key words: Calendula officinalis, dry extract, antioxidant activity, hydrophilic cream, preparation, evaluation.