Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Methoxy-flavones identified from Ageratum conyzoides induce capase -3 and -7 activations in Jurkat cells

Felix Acheampong
  • Felix Acheampong
  • Department of Chemistry and Biochemistry, South Dakota State University, Brookings, SD57007, USA.
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John Reilly
  • John Reilly
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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Christopher Larbie
  • Christopher Larbie
  • Department of Biochemistry and Biotechnology, KNUST, Kumasi-Ghana.
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Matthew Spencer
  • Matthew Spencer
  • Regulus Therapeutics, 3545 John Hopkins Court, Suite 210 San Diego, CA 92121-1121, USA.
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Karl Gunderson
  • Karl Gunderson
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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Regina Appiah-Opong
  • Regina Appiah-Opong
  • Noguchi Memorial Institute of Medical Research, UG, Accra-Ghana.
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Christopher Howson
  • Christopher Howson
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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Jennifer Porier
  • Jennifer Porier
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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Kellie Joyce
  • Kellie Joyce
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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Valentina Jeliazkova
  • Valentina Jeliazkova
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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Sarah Voytek
  • Sarah Voytek
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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Carol Ginsburg-Moraff
  • Carol Ginsburg-Moraff
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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Stefan Thibodeaux
  • Stefan Thibodeaux
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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Jill Kublbeck
  • Jill Kublbeck
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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Steven Austin
  • Steven Austin
  • Novartis Institutes for Bio-Medical Research Incoporation, Cambridge, MA 02139, USA.
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  •  Received: 27 July 2017
  •  Accepted: 14 September 2017
  •  Published: 10 October 2017

Abstract

New therapies for leukemia are urgently needed due to adverse side effects, tumor resistance and lack of selectivity of many chemotherapeutic agents in clinical use. Ageratum conyzoides has been used in folklore medicine for managing leukemia and other cancers. Thus, this study aimed to investigate the effects of fractions, sub-fractions and purified compounds from the ethanol leaf extracts of A. conyzoides against Jurkat cells-model for acute T cell leukemia. A two-dimensional purification process using normal phase flash, followed by reverse phase purification was necessary to isolate pure methoxy-flavones, which were further characterized by Nuclear Magnetic Resonance (NMR) and MS-MS. The effect of fractions or pure compounds on cell viability was determined using either the MTT reagent or CellTiter-Blue® assay, while the caspase-3 and -7 activation was tested with Caspase-Glo® 3/7 assay. Prediction of compounds’ drug disposition profiles in vivo was measured with biomimetic affinity chromatography methodologies.

 

Key words: Ageratum conyzoides, methoxy-flavones, Jurkat, biomimetic affinity chromatography, cell viability.