Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3758

Full Length Research Paper

Quality evaluation of different products derived from Ganoderma

Jing Yang1,2, Yiwen Chen1,2#, Nga-I Leong1,2, Jing Zhao1,2#, Jin-ao Duan3, Yu-ping Tang3, Shao-ping Li1,2,3*
1State Key Laboratory of Quality Research in Chinese Medicine, Macao SAR, P. R. China. 2Institute of Chinese Medical Sciences, University of Macau, Macao SAR, P. R. China 3Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, P. R. China.
Email: [email protected]; [email protected]

  •  Accepted: 01 February 2012
  •  Published: 16 March 2012

Abstract

Quality assessment of commercial products derived from Lingzhi was performed based on both their small (triterpenes) and macro (polysaccharides) molecular bioactive components by using high-performance thin-layer chromatography (HPTLC) and high-performance size-exclusion chromatography-evaporative light scattering detector (HPSEC-ELSD). Enzyme (α-amylase) digestion was employed for avoiding the interference, induced by starch added in formula preparation, on the analysis of polysaccharides during HPSEC-ELSD analysis. The results showed the quality variation among Lingzhi products were obvious. The developed method is simple, rapid, reliable and suitable for evaluation of quality of Lingzhi and their products.

 

Key words: Ganoderma, polysaccharides, triterpenes, high-performance thin-layer chromatography (HPTLC), high-performance size-exclusion chromatography (HPSEC