Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3778

Full Length Research Paper

Chemical composition and antitumor activity of polysaccharide from Inonotus obliquus

Lixia Zhang1, 2, Cong Fan1, Songchen Liu 1, Zhenfeng Zang 1, Lili Jiao3 and Liping Zhang1*
1Laboratory of Biochemistry and Molecular Biology, School of Life Science, Northeast Normal University, Changchun 130024, China. 2School of Life Science, Daqing Normal College, Daqing 163712, China. 3Ji Lin Ginseng Academy, Chang Chun University of Chinese Medicine, Changchun 130017, China.
Email: [email protected]

  •  Accepted: 11 February 2011
  •  Published: 04 April 2011

Abstract

The alkaline soluble polysacchride AIOPA isolated and purified from Inonotus obliquususing gel filtration was subjected to composition analysis and determined for the antitumor and immunomodulatory activitives. Based on the results of high performance size-exclusion chromatography (HPSEC), gas chromatography (GC) and infra-red (IR) spectrum, AIOPA consisted of rhamnose, xylose, manose, galactose, glucose and galacturonic acid in a molar ratio of 3.09:1.61:2.06:4.45:19.7:1 with a molecular weight of 6.3 kDa. In the present study, it was found that AIOPA remarkably enhanced spleen and thymus index in mice bearing S180 sarcoma, and also stimulated LPS-induced splenocyte proliferation. Immunomodulatory activity assay in vitro indicated AIOPA could significantly enhance cellular lysosomal enzyme activity, nitric oxide (NO) formation and tumor necrosis factor-a (TNF-α) secretion in macrophages. Furthermore, AIOPA dose-dependently stimulated macrophages to produce NO through the up-regulation of inducible NO synthase (iNOS) activity and the maximal effect occurred at a concentration of 300 ug/ml by AIOPA.These data suggest that the antitumor activity of AIOPA may be associated with its potent immunostimulating effect. 

 

Key words: Inonotus obliquus, polysaccharide, immunomodulatory, antitumor.