Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3761

Full Length Research Paper

Essential oil composition and antimicrobial activities of aerial parts from Tunisian Anacyclus clavatus (Desf.)

Saoussen Hammami1, Abdelkader Ben Salem1, Maha Mastouri2, Danilo Falconieri3,4, Mohamed Gorcii5, Mohamed Farouk M’henni1, Bruno Marongiu3 and Zine Mighri1*    
1Unit Recherche12-04, Applied Chemistry and Environment, Faculty of Sciences of Monastir, 5000 Monastir, Tunisia. 2Laboratory of Bacteriology, University Hospital F. Bourguiba, 5000 Monastir, Tunisia. 3Department of Chemistry, University of Cagliari, Cittadella Universitaria of Montserrat, 09042, Cagliari, Italy. 4M. Technical Institute Giua, via Monte Cassino, Cagliari, Italy. 5 Laboratory of Parasitology-Mycology, University Hospital F. Bourguiba, 5000 Monastir, Tunisia.      
Email: [email protected]

  •  Accepted: 16 July 2012
  •  Published: 10 January 2013


Volatiles composition of aerial parts from medicinal Anacyclus clavatus (Asteraceae) was determined by gas chromatography-flame ionization detector (GC/FID) and gas chromatography-mass spectrometry (GC/MS) analysis. Altogether, 46 compounds were identified representing 81.2% of the total volatiles. The main components were Trans-Chrysanthenyl acetate (12.3%), Cis-Thujone (9.8%), Chrysanthenone (8.2%) and Trans-Thujone (7.3%). The volatile oil was screened for antibacterial and anti-Candida activities using dilution and disc diffusion methods. Thus, the studied fraction showed moderate activities against four Candida species and was most effective against Gram-negativePseudomonas aeruginosa.


Key words: Anacyclus clavatus (Desf.), aerial parts, steam distillation, gas chromatography-mass spectrometry (GC/MS), antimicrobial effects.