Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3564

Full Length Research Paper

Effects of an aqueous extract of Three Ballerina on the survival of Escherichia coli AB1157 cultures and in the action of stannous chloride

Pinto N. S.1, Carmo F. S.2, Diniz C. L.3, Almeida D. S.3, Pereira M. O.1, Santos-Filho S. D.3*, Medeiros A. C.1 and Bernardo-Filho M.3,4
1Programa de Pós-Graduação em Ciências da Saúde da Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil. 2Programa de Pós-Graduação de Ciências Médicas da Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil. 3Laboratório de Radiofarmácia Experimental, Departamento de Biofísica e Biometria, Instituto Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil. 4Controladoria de Pesquisa, Instituto Nacional do Câncer, Rio de Janeiro, RJ, Brasil.
Email: [email protected]

  • Article Number - ABF271D19309
  • Vol.5(14), pp. 3256-3259, July 2011
  •  Accepted: 01 April 2011
  •  Published: 18 July 2011


The global interest in natural substances has increased. Three Ballerina (TB) is a natural formula that has medicinal properties. Scientific information about this formula is not found in the PubMed and this fact has stimulated investigations about its biological effects. An experimental model using a reducing agent, stannous chloride (SnCl2), was carried out. Although, SnCl2 is used in nuclear medicine to obtain technetium-99 m radiopharmaceuticals, it is capable to generate free radicals. The aim of this work was to evaluate the biological effects of an aqueous extract of TB on the survival of Escherichia coli (E. coli) AB1157 (wild type) cultures and to verify if this extract protect a E. coli culture against the action of SnCl2E. coli AB1157 cultures (exponential growth phase) were incubated with TB extract (23.4 mg/ml) or 0.9% NaCl solution as a control. E. coli cultures were also incubated with SnCl2 (25 µg/ml) + TB extract (23.4 mg/ml) or SnCl2 (25 µg/ml) alone. Aliquots from these treatments were spread onto Petri dishes containing solidified LB medium. The colony-forming units (CFU) were counted after overnight and the survival fractions calculated. Data reveal that the TB did not alter the survival of the E. coli culture, however, it has protected the cells against the lethal effect of the SnCl2. In conclusion, the TB extract did not present cytotoxic effects, but it appears to have redox action.


Key words: Escherichia coli, three ballerina, stannous chloride, cytotoxic effect.

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