Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Investigation of the inhibitory effects from the Brazilian medicinal plant Pothomorphe umbellata L. (Piperaceae) on the molecular pathways of cyclooxygenase-2 and nuclear factor kappa B

Andrey Pereira Lopes
  • Andrey Pereira Lopes
  • Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema, São Paulo, Brazil.
  • Google Scholar
Bianca Souza Bagatela
  • Bianca Souza Bagatela
  • Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema, São Paulo, Brazil.
  • Google Scholar
Fernando Luiz Affonso Fonseca
  • Fernando Luiz Affonso Fonseca
  • Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema, São Paulo, Brazil.
  • Google Scholar
Edson Luis Maistro
  • Edson Luis Maistro
  • Faculty of Sciences, State University of São Paulo, Marilia, São Paulo, Brazil.
  • Google Scholar
Jairo Kenupp Bastos
  • Jairo Kenupp Bastos
  • Faculty of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
  • Google Scholar
Dhammika Nanayakkara
  • Dhammika Nanayakkara
  • National Center for Natural Products Research, the University of Mississippi, Oxford, Mississippi, USA.
  • Google Scholar
Fábio Ferreira Perazzo
  • Fábio Ferreira Perazzo
  • Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema, São Paulo, Brazil.
  • Google Scholar


  •  Received: 19 April 2016
  •  Accepted: 01 August 2016
  •  Published: 17 August 2016

Abstract

Presently, medicinal plants, such as Pothomorphe umbellata L. (Piperaceae), have been assessed as sources of potential therapeutic medicines. The inhibitory effects of the crude extract, fractions and 4-nerolidylcathecol, a phenolic compound which has been assigned significant activity in several beneficial properties performed by the plant, on the molecular pathways of cyclooxygenase-2 (COX-2) and nuclear factor kappa B (NF-κB) were evaluated. The in vitro inhibition of COX-2 was performed by enzyme-linked immunosorbent assay (ELISA). Methylene chloride fraction demonstrated the preeminent inhibition of COX-2. In order to estimate the inhibition of NF-κB, an adaptation of the luciferase plasmid assay was developed. 4-Nerolidylcathecol presented the best inhibition NF-κB activity. The results obtained from the in vitro assays were promising, mainly on the molecular pathway of NF-κB, once 4-nerolidylcathecol demonstrated a remarkable inhibition activity.

Key words: Piperaceae, Pothomorphe umbellata, Piper umbellatum, 4-nerolidylcathecol, folk medicine, cyclooxygenase-2 (COX-2), nuclear factor-kappaB (NF-kappaB), inflammation.