Anemarrhena rhizome known as “Zhi-Mu” is an important traditional Chinese medicine (TCM). In this study, an accurate, rapid and reproducible high performance capillary electrophoresis (HPCE) method with ultraviolet (UV) detection has been established for the simultaneous determination of two major bioactive xanthone glycosides, mangiferin and neomangiferin in rhizome of Anemarrhena asphodeloides (Zhi-Mu). The running buffer was 30 mM borate (pH 9.3) containing 15% methanol. The separation voltage was 30 kV and temperature was 25°C. Gentiopicroside was adopted as an internal standard (IS) and detection wavelength was 254 nm. Within 15 min, the contents of mangiferin and neomangiferin can be simultaneously assayed. The results also indicate that it is important to assay the xanthone content in quality control of “Zhi-Mu” for its great variation among different samples.
Key words: High performance capillary electrophoresis (HPCE), Anemarrhena asphodeloides, mangiferin, neomangiferin.
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