This study aimed to evaluate anti-arthritic and cytotoxic effects of methanolic extract of Ixora nigricans leaf. Anti-denaturation method was performed by using bovine serum albumin (BSA) to evaluate the anti-arthritic potential. The screening of cytotoxic activity was done using brine shrimp lethality bioassay. The in vitro study on Ixora nigricans leaf showed the presence of significant anti-arthritic activity. Here, the Ixora nigricans leaf showed 79.35% at 1000 ug/ml and 46.77% at 31.25 µg/ml concentration, whereas the standard drug showed 85.49% at 1000 ug/ml and 51.61% at 31.25 µg/ml concentration. Moderate cytotoxicity was found for methanolic extract and it was compared with the standard drug vincristine sulfate in the brine shrimp bioassay. In the present study, the LC50 values of methanolic crude extract of Ixora nigricans leaf and vincristine sulfate were 179.18 and 12.59 µg/ml, respectively. The results of this study demonstrated that methanolic extract of I. nigricans leaf contains significant anti-arthritic activity and moderate cytotoxic activity.
Keywords: Ixora nigricans, cytotoxicity, brine shrimp, anti-arthritic, inhibition, protein denaturation.
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