Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Comparative antioxidant analysis of hexane extracts of Terminalia chebula Retz. prepared by maceration and sequential extraction method

Harpreet Walia1, Subodh Kumar2 and Saroj Arora1*
1Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar-143005, Punjab, India. 2Department of Chemistry, Guru Nanak Dev University, Amritsar-143005, Punjab, India.
Email: [email protected]

  •  Accepted: 26 February 2010
  •  Published: 04 July 2011


The aim of this work is to compare the antioxidant efficacy and the phenolic content of two hexane extracts viz. ‘Hex 1’ and ‘Hex 2’ of fruits of Terminalia chebula prepared by maceration and sequential method, respectively. The extracts were tested for their relative levels of antioxidant activity and the total phenolic content using DPPH, deoxyribose, reducing power, chelating power, lipid peroxidation and Folin-Ciocalteu method. Furthermore, the UV–VIS spectrum of extracts and the correlation between total phenolic content were examined in order to give an orientation to the search of phytochemicals responsible for their activity. From the results, it was concluded that phenolic compounds were predominant in the ‘Hex 2’ prepared by sequential extraction method. The antioxidative potential of ‘Hex 2’ was also far superior to the ‘Hex 1’ prepared by maceration method. Such study would contribute to further knowledge relating to the extraction of plant materials by different methods.


Key words: Terminalia chebula, extraction method, antioxidant activity, phenolic compounds.