Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3778

Full Length Research Paper

Antioxidant and hepatoprotective effects of the internal layer of oak fruit (Jaft)

Mehdi Akbartabar Toori1, Mahsa Mirzaei2, Nooshin Mirzaei3, Prasad Lamrood4 and Ali Mirzaei1*    
1Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. 2College of Pharmacy, Sinhgad, 44/1, Vadgaon, Sinhgad Road, Maharashtra, India. 3Fergusson Colleges, Pune- 411004, Maharashtra, India. 4Department of Botany, Ahmednagar College, Ahmednagar – 414001, Maharashtra, India.    
Email: [email protected]

  •  Accepted: 04 January 2012
  •  Published: 03 January 2013


An aqueous extract of the internal layer of oak fruit (Jaft) was evaluated for in vitroantioxidant activity and hepatoprotective effects on carbon tetrachloride (CCl4)-induced liver damage in rats. Thirty-six male Wistar rats were randomly divided into 6 groups. Groups 1, 2 and 3 served as negative controls and were given olive oil, distilled water, and Jaft extract (500 mg/kg), respectively. Group 4 served as the toxic group and received 1 ml/kg CCl4intraperitoneally as a single dose every 72 h. Groups 5 and 6 received oral aqueous extracts of Jaft at 250 and 500 mg/kg, respectively, 1 h after the injection of CCl4 for every 72 h. Jaft doses of 250 and 500 mg/kg resulted in decreased serum enzyme and bilirubin levels (P < 0.01), which is an indication of hepatoprotection. CCl4 induced a significant increase in aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), and total bilirubin and a reduction of total protein (P < 0.01). Treating rats with Jaft extract significantly lowered these serum marker enzymes as compared to CCl4-treated rats (P < 0.001). Aqueous extracts of Jaft at 250 and 500 mg/kg showed hepatoprotective potential and did not have toxic effects.


Key words: Antioxidant activity, hepatoprotective, enzyme markers, oak fruit.