Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3702

Full Length Research Paper

Karyotype analysis of obtained tetraploid in medicinal plant (Platycodon grandiflorus)

Fuhong Yang
  • Fuhong Yang
  • Pingliang Academy of Agricultural Science, Pingliang Gansu, 744000, China.
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Lingjiao Li
  • Lingjiao Li
  • College of Agriculture, Shanxi Agricultural University, Taigu Shanxi, 030801, China.
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Erhua Rong
  • Erhua Rong
  • College of Agriculture, Shanxi Agricultural University, Taigu Shanxi, 030801, China.
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Yongbo He
  • Yongbo He
  • Horticulture Institute of Shanxi Agricultural Academy, Shanxi Taiyuan, 030031, China.
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Xiaoming Zhao
  • Xiaoming Zhao
  • College of Agriculture, Shanxi Agricultural University, Taigu Shanxi, 030801, China.
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Yuxiang Wu*
  • Yuxiang Wu*
  • College of Agriculture, Shanxi Agricultural University, Taigu Shanxi, 030801, China.
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  •  Received: 08 February 2015
  •  Accepted: 26 February 2015
  •  Published: 03 March 2015

Abstract

Platycodon grandiflorus is an important medicinal plant in China. Induction of polyploidy was successfully attempted in this medicinal plant using 0.05% colchicine semi-solid under 72 h by apical shoot tip treatment of young seedlings, and then 50% mutant plants were obtained. It was testified by the chromosome number in mitosis and its karyotype analysis for diploid control and obtained mutants. The results showed that the chromosome number of the diploid plant was 2n=2x=18, whereas the chromosome number of the obtained mutants was 2n=4x=36. The karyotype formula of mutant plant and diploid control were K=2n=4X=36=24m+12sm (4SAT) and K=2n=2X=18=12m+6sm (2SAT), respectively by karyotype analysis and they belonged to 2B and 2A karyotype. It was confirmed that the obtained mutant plants were tetraploid.

 

Key words: Platycodon grandiflorus, Obtained mutants, karyotype analysis.