Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3753

Full Length Research Paper

In vitro analysis of Cupressus sempervirens L. plant extracts antibaterial activity

Hassan Javed Chaudhary1*, Warda Shahid1, Asghari Bano1, Farman Ullah2, Farooq Munis1, Shah Fahad1 and Izhar Ahmad1
1Department of Plant Sciences, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, Pakistan. 2Institute of Medical Sciences, Kohat University of Science and Technology, Kohat, Pakistan.
Email: [email protected]

  •  Accepted: 27 October 2011
  •  Published: 31 January 2012


The antibacterial activity of methanolic, ethanolic and ethyl acetate extracts of leaf ofCupressus sempervirens L. was determined on six bacteria, namely Staphylococcus aureus,Bacillus subtilisPseudomonas aeruginosaEscherichia coliKlebsiella pneumoniae andSalmonella typhimurium using agar well diffusion method. Among the plant extracts, a significant antimicrobial activity was obtained by methanolic extracts followed by the ethyl acetate and ethanol extracts. The methanolic extract exhibited maximum inhibitory activity against K. pneumonia, B. subtilis and S. aureus. The ethanolic extract showed higher activity against P. aeruginosa. Greater inhibitory activity against S. typhimurium and E. coliwas possessed by ethyl acetate extract of C. sempervirens. This study revealed that the C.sempervirens exhibited greater inhibitory activity against the tested bacterial strains and can be used in the cure of pathogenic diseases and improvement of crop growth.


Key words: Extracts, tannins, berberine, Cupressus sempervirens L., agar.


 DMSO, Dimethyl sulfoxide.