The antibacterial activity of methanolic, ethanolic and ethyl acetate extracts of leaf ofCupressus sempervirens L. was determined on six bacteria, namely Staphylococcus aureus,Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae andSalmonella typhimurium using agar well diffusion method. Among the plant extracts, a significant antimicrobial activity was obtained by methanolic extracts followed by the ethyl acetate and ethanol extracts. The methanolic extract exhibited maximum inhibitory activity against K. pneumonia, B. subtilis and S. aureus. The ethanolic extract showed higher activity against P. aeruginosa. Greater inhibitory activity against S. typhimurium and E. coliwas possessed by ethyl acetate extract of C. sempervirens. This study revealed that the C.sempervirens exhibited greater inhibitory activity against the tested bacterial strains and can be used in the cure of pathogenic diseases and improvement of crop growth.
Key words: Extracts, tannins, berberine, Cupressus sempervirens L., agar.
DMSO, Dimethyl sulfoxide.
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