Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3714

Full Length Research Paper

Purification, chemical characterization and in vitro antioxidant activities of alkali-extracted polysaccharide fractions isolated from the fruits of Schisandra chinensis

Yu Sheng1, Yang Liu1, Xiaodong Huang2, Guangxin Yuan3 and Ming Guan1*
1School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China. 2Jilin Medical College, Jilin 132013, China. 3Pharmaceutical College of Beihua University, Jilin 132013, China.
Email: [email protected], [email protected]

  •  Accepted: 18 August 2011
  •  Published: 30 October 2011

Abstract

The alkali-extracted crude polysaccharide was extracted from the fruits of Schisandra chinensis, and further purified by DEAE-cellulose and Sepharose CL-6B chromatography, giving three polysaccharide fractions coded as ASPS-a-1, ASPS-b-2 and ASPS-b-3. In this study, their chemical and physical characteristics of polysaccharide fractions and antioxidant capacity, including scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, chelating ability, hydroxyl radicals and superoxide radicals, were evaluated. The results showed that ASPS-b-3 exhibited significantly antioxidant activity at a concentration-dependent manner. The alkali-extracted polysaccharide fraction can be developed to be new antioxidant drug.

 

Key words: Schisandra chinensis, polysaccharide, antioxidant activity.