Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Immunoregulatory activity of root bark of Cassia sieberiana D.C. in a modified adjuvant-induced arthritis in rat

Kofi Donkor
  • Kofi Donkor
  • Department of Pharmacology/Toxicology, Centre for Plant Medicine Research, P. O. Box 73, Mampong-Akuapem, Ghana.
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Eric Woode
  • Eric Woode
  • Department of Pharmacology, Kwame Nkurumah University of Science and Technology, Kumasi, Ghana.
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Laud Kenneth Okine
  • Laud Kenneth Okine
  • Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon, Ghana.
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  •  Received: 22 August 2017
  •  Accepted: 03 October 2017
  •  Published: 17 October 2017

Abstract

The present study seeks to evaluate the immunoregulatory effects of extracts of the root bark of Cassia sieberiana, a plant used in Ghana for various painful inflammatory conditions, in a modified adjuvant arthritis model induced by administration of very low dose of Mycobacterium tuberculosis (MT) – carrageenan mixture in the rat. A volume of 0.1 mg kg-1 heat killed MT in paraffin oil was mixed with equal volume of 0.05% (w/v, normal saline) carrageenan. A single intraplantar dose of 0.1 ml of the MT - carrageenan mixture was administered to experimental animals. Groups were administered extracts (20 to 200 mg kg-1, p.o.), dexamethasone (0.3 mg kg-1, p.o.) or vehicle an hour prior to the test and daily from test day till the 6th day. Paw volume (ml) of the injected hind limbs were measured using a plethysmometer, while paw withdrawal thresholds were determined using an analgesy meter. Serum levels of IL-1α, IL-6, IL-10 and TNF-α were determined via enzyme linked immunosorbent assay (ELISA). Results showed that the extracts attenuated the inflammation and hyperalgesia caused by the intraplantar injection of MT-carrageenan mixture in the rats in a dose-dependent fashion. Similarly, the extracts reduced the serum levels of IL-1α, IL-6 and TNF- α while increasing the levels of IL-10. It can be concluded that the anti-inflammatory activity of extracts of root bark of C. sieberiana may be attributable to their immunomodulatory effects via suppression of pro-inflammatory cytokines, TNF-α, IL-1α and IL-6; and elevation of the anti-inflammatory cytokine, IL-10 levels, in serum.

Key words: Immunoregulatory, pro-inflammatory cytokines, TNF-α, adjuvant, C. sieberiana, IL-10.