Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Solanum jabrense Agra & M. Nee (Solanaceae) exhibits spasmolytic activity on guinea‑pig ileum

Fabiana de Andrade Cavalcante1*, Joelmir Lucena Veiga da Silva2, Antonilêni Freire Duarte Medeiros3, Fladmir de Sousa Claudino4, Maria de Fátima Agra3,5, Tania Maria Sarmento da Silva6, Mário Geraldo de Carvalho7, Raimundo Braz-Filho8 and Bagnólia Araújo da Silva3,5    
1Departamento de Fisiologia e Patologia, CCS, UFPB, Brazil. 2Departamento de Saúde, Farmácia-Bioquímica, UNINOVE, Brazil. 3Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, CCS, UFPB, Brazil. 4Departamento de Biologia Molecular, CCEN, UFPB, Brazil. 5Departamento de Ciências Farmacêuticas, CCS, UFPB, Brazil. 6Departamento de Ciências Moleculares, UFRPE, Brazil. 7Departamento de Química, ICE, UFRRJ, Brazil. 8Setor de Química de Produtos Naturais, LCQUI, CCT, UENF, Brazil.    
Email: [email protected]

  •  Accepted: 24 August 2012
  •  Published: 03 April 2013

Abstract

Some Solanum species are commonly known as “jurubeba” and have a variety of biological activities such as hypotensive, antidiarrheal, and spasmolytic actions. Solanum jabrense is a perennial shrub or tree from Solanaceae family. The hexane extract from aerial parts of S. jabrense (SJ-Hex) was assayed for possible spasmolytic effect in guinea-pig ileum. SJ-Hex inhibited the contractions induced by carbachol (IC50=36.0±6.2 µg/ml) or histamine (IC50=37.3±6.1 µg/ml) in a concentration dependent and equipotent manner. SJ-Hex also antagonized cumulative concentration-response curves elicited by histamine. The characterization of the blockade was evaluated by the observation that SJ-Hex shifted to the right cumulative concentration-response curves to histamine in a reversible and non-competitive manner. KCl-, carbachol- and histamine-induced tonic contractions were also inhibited (IC50=51.9±11.4; 32.7±11.5, and 31.1±5.3 µg/ml) in a concentration-dependent and reversible manner, suggesting that SJ-Hex could be acting on voltage-operated Ca2+channels. This hypothesis was confirmed by the observation that SJ-Hex inhibited in a concentration-dependent manner CaCl2-induced contractions in depolarizing medium. These results suggest that SJ-Hex produces spasmolytic action in guinea-pig ileum probably by inhibiting the Ca2+ influx through voltage-operated Ca2+ channels.

 

Key words: Solanum jabrense, Solanaceae, spasmolytic activity, Ca2+ channels.