Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3728

Full Length Research Paper

Development and validation of a new method to quantify vitexin-2''-O-rhamnoside on Passiflora L. extracts

Lorenna C. da Rosa
  • Lorenna C. da Rosa
  • Laboratório de Toxicologia Ambiental, Departamento de Ciências Biológicas, Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz, Rio de Janeiro RJ, Brazil.
  • Google Scholar
Monica R. P. Siqueira
  • Monica R. P. Siqueira
  • Laboratório de Toxicologia Ambiental, Departamento de Ciências Biológicas, Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz, Rio de Janeiro RJ, Brazil.
  • Google Scholar
Francisco J. R. Paumgartten
  • Francisco J. R. Paumgartten
  • Laboratório de Toxicologia Ambiental, Departamento de Ciências Biológicas, Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz, Rio de Janeiro RJ, Brazil.
  • Google Scholar
Georgia Pacheco
  • Georgia Pacheco
  • Núcleo de Biotecnologia Vegetal, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, PHLC, sala 505 – Maracanã, Rio de Janeiro, Brazil.
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Elisabeth A. Mansur de Oliveira
  • Elisabeth A. Mansur de Oliveira
  • Núcleo de Biotecnologia Vegetal, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, PHLC, sala 505 – Maracanã, Rio de Janeiro, Brazil.
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Davyson de L. Moreira
  • Davyson de L. Moreira
  • Departamento de Produtos Naturais, Instituto de Tecnologia em Fármacos, Fundação Oswaldo Cruz, Rio de Janeiro RJ, Brazil.
  • Google Scholar


  •  Received: 22 September 2020
  •  Accepted: 18 December 2020
  •  Published: 31 January 2021

Abstract

The genus Passiflora L. is the most representative of the Passifloraceae family and includes about 500 species. The aim of this work was to analyze three different passion fruit species in relation to vitexin-2´´-O-rhamnoside content by high-performance liquid chromatography coupled to a diode-array ultaviolet detector. Samples were prepared by water infusion (10% w/v), dried by lyophilization, and stored in amber vials at -20°C. The method for quantification of vitexin-2´´-O-rhamnoside was developed and validated using a Shimadzu Class-VP liquid phase chromatograph. Quantification of vitexin-2´´-O-rhamnoside was done with an Ascentis-phenyl supelco column (250 mm x 4.6 mm i.d. x 5 µm), mobile phase composed of ultrapure water (pH = 3.0) (Solvent A)/ acetonitrile (Solvent B), in gradient elution mode, flow rate of 1.4 mL/min, and UV detection at 340 nm. The adopted method showed great linearity, precision, accuracy, detection (LOD), and quantification (LOQ) limits, recovery and robustness. Total analysis time was 16 min. This method has clear advantages when compared to those found in the literature, since the use of a silica-based phenyl column allowed the best chromatographic resolution, resulting in better LOD and LOQ. Vitexin-2''-O-rhamnoside content was higher in P. foetida (7.21%), followed by P. setacea (3.66%), and P. alata (2.89%).

Key words: Vitexin-2''-O-rhamnoside quantity, high-performance liquid chromatography (HPLC) new method, passion fruit.