Medicinal plants used for the management of hepatic ailments in Katsina State , Nigeria

People in Katsina State, Nigeria have been using medicinal plants to cure several ailments associated with liver since time immemorial; however the use of such plants was never documented. In this study, an ethnobotanical survey was conducted to document the medicinal plants used for the management of hepatic ailments in Katsina State, Nigeria. A semi-structured questionnaire method was adopted to interview 150 respondents (50 respondents from 1 Local Government Area of each of the three Senatorial Zones of the State) comprising herbalist, farmers, house wives, and others. A total of 62 plant species belonging to 57 genera distributed among 34 families were documented. Most of the reported plants belong to the Fabaceae (24.19%), Moraceae (6.45%), followed by Anacardiaceae, Euphorbiaceae and Asteraceae (each with 4.84%). Senna occidentalis L., Ficus thonningii Bl., and Moringa oleifera Lam. had the highest relative frequency of citation (RFC) of 0.75, 0.64, and 0.53 respectively. Majority (38.71%) of the reported plants were trees and about 79.03% of the surveyed plants are sourced from wild. Leaves were the most frequently used (45.16%) plants part. Most of the herbal medicines (80.65%) were prepared in form of decoction and all the medicines were administered orally. This is the first ethnobotanical study on hepatic ailments in the study area. Results of the study could serve as baseline data based on which further ethnopharmacological investigations would be carried out. Further researches aimed at conserving as well as validating the folkloric use of the surveyed plants would be ideal.


INTRODUCTION
Liver, the second-largest organ in the human body, plays a key role in the metabolism of various substances.Besides that, liver also performs vascular, immunological, secretory as well as excretory functions in the human body system (Mitra and Metcalf, 2009).Liver is involved in almost all the biochemical pathways to growth, fight against disease, nutrient supply, energy provision and reproduction (Ward and Daly, 1999).Unfortunately *Corresponding author.E-mail: sulaiman.kankara@umyu.edu.ng.
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License however, liver is being affected by both infectious and non-infectious diseases.Liver diseases pose a serious challenge to international public health (Ahsan et al., 2009).One of the most common causes of liver disease is inflammation, which often results from abuse of alcohol, poor diet or even malnutrition.Nigeria is one of the countries with largest burden of hepatitis B virus (one of the leading causative agents of hepatic ailments) with 10-15% prevalence (Owolabi and Ojo, 2008).
Unfortunately, conventional or synthetic drugs used in the treatment of liver diseases are inadequate and sometimes can have serious side effects (Rao et al., 2006).Moreover, there are still no specific treatments in modern medicine that give protection to the liver against damage or help to regenerate hepatic cells (Chatterjee, 2000;Chattopadhyay, 2003).
The scientific study of the relationship that exists between people and plants is called ethnobotany (Ijaz et al., 2017).Since the beginning of human civilization, medicinal plants have been used by mankind for its therapeutic value.Today about 80% of the world's population rely predominantly on plants and plant extracts for healthcare (Setzer et al., 2006).Nature has been a source of medicinal agents for thousands of years and an impressive number of modern drugs have been isolated from natural sources.Many of these isolations were based on the uses of the agents in traditional medicine.In Nigeria, many rural communities have been using medicinal plants to cure various forms of ailments (Sani and Aliyu, 2011).
Recently, the use of medicinal plants to cure various forms of liver diseases and dysfunctions is becoming increasingly popular and has received wide acceptance (Oyagbemi and Odetola, 2010).Moreover, a large number of medicinal plants and compounds derived from them have been found to have some hepatoprotective ability (Kalaskar and Surana, 2014;Mishra et al., 2014;Arka et al., 2015;Xu et al., 2018).
The use of medicinal plants as traditional medicine is well known in rural areas of many developing countries.Traditional healers claim that their medicine is cheaper and more effective than modern medicine.Indigenous knowledge on the use of medicinal plants is transmitted orally in various communities of the world.This lead to fast disappearing of the knowledge due to the advent of modern technology and transformation of traditional culture and the younger generations are not interested in carrying on this tradition.
There is therefore, a great danger that this knowledge of traditional medicine could be lost.Katsina is one of the poor states of Nigeria.Most people in the area depend on medicinal plants for their healthcare because of poverty as well as affordability and accessibility of the medicinal plants (Kankara et al., 2015).Despite the intense use of the plants, no attempt was made to document their usage for hepatic ailments.It is against this background this research was designed with the aim of documenting medicinal plants used for the management of hepatic ailments in the study area.

Data collection
This research work was conducted during the period of August 2016 to October 2016 in three Senatorial Zones of Katsina State, Nigeria.One local government area was randomly selected from each Senatorial Zone; the Local Government Areas were Batagarawa, Kankia, and Malumfashi.The ethnomedicinal plants data were gathered using a semi-structured questionnaire by interviewing 150 respondents (50 respondents from 1 Local Government Area of each of the Senatorial Zones of the State).The questionnaire was validated using Cronbach's alpha with 0.5 < α ≥ 0.8 degree of consistency.During the interview, questions asked according to the questionnaire were divided into two parts, A and B. In part A, socio-demographic information of the respondents was recorded, and information of the plants used for the management of hepatic ailments was recorded in B part.

Collection and identification of plants specimens
Six field trips were carried out to collect the specimens of the reported plants from their natural habitat and cultivated lands with the help of some medicinal plants collectors, traditional healers and farmers.Flora collection permit was obtained from Local Authorities before embarking on the trips.Photographs of the plants were taken using a Sony (14.0) digital camera to aid in the botanical authentication of the plants.Identification of the reported plants was achieved by the aid of herbarium specimens deposited at Umaru Musa Yar'adua University Herbarium and literature on medicinal plants found in Nigeria.Further identifications of the surveyed plants were obtained using catalogue of life (2016) plants databases available online.Herbarium specimens were prepared and deposited in the Herbarium of Umaru Musa Yar'adua University, Katsina State.

Data analysis
A descriptive statistical method using percentages and frequencies was used to analyze the socio-demographic data of the respondents, and the results of this study were analyzed using the Relative Frequency of Citation (RFC).RFC is used to determine the relative importance of a particular species.It is determined using the relation: RFC = Fc/N, where Fc is the number of respondents who cited a particular species and N is the total number of the respondents (Tardio and Pardo-de-Santayana, 2008).

Socio-demographic information
Table 1 shows the socio-demographic information of the respondents.As shown in the table, 57 respondents were males (38%) and 93 respondents were females (62%)among the total number of 150 respondents interviewed during the survey.Majority of the respondents (52%) are 56 to 65 years of age among the different age groups interviewed.Most of the respondents (78%) had no formal education, and 46% were herbalists.The research revealed that most of the respondents to the indigenous knowledge with regard to their age show that majority were of old age (Figure 1).This reveals that, the passage of ethnomedicinal knowledge is probably more from the elders to the younger ones as similarly reported by Adekunle (1992), and also knowledge transferred to the younger generation was very poor; they seem to keep the knowledge with them either for the sake of secrecy or due to apathy of the younger generation to traditional knowledge.This, however, poses a serious threat to the indigenous knowledge because it may eventually be lost forever following the demise of the older generation (Kankara et al., 2015).

Plant species used for liver diseases
Information on the medicinal plants used for the management of liver diseases in the study area is presented in Table 2.The table contains all the surveyed plants, their common names, scientific names, family names, parts of the plant used, growth habit, domestications, frequency of citation as well as modes of preparation and the routes of administration.A total of 62 plant species belonging to 57 genera, distributed among 34 families are used to treat various liver diseases in Katsina State, Nigeria.Senna occidentalis appeared to be the most popular specie in this study.Phytochemicals present in this plant (including but not limited to flavonoids, alkaloids, lignins, tannins and phenols) may be responsible for its curative power (Manikandaselvi et al., 2016).Several biological activities of S. occidentalis (formerly known as Cassia occidentalis) such as antimicrobial (Hussaini and Deeni, 1991), antimalarial ( Tona et al., 2001), anti-inflammatory (Sadique et al., 1987) and anticarcinogenic (sharma et al., 1999) are reported.It is also interesting to note that hepatorotective effect of S. occidentalis has been proven scientifically (Jafri et al., 1991;Usha et al., 2007).It is also worth noting that some of the species reported in this study such as Hibiscus sabdariffa, Moringa oleifera and Zingiber officinale are also used for the same purpose elsewhere (Panday, 2011).The highest recorded family is Fabaceae with 15 species, followed by Moraceae with 4 species, and Anacardiaceae, Asteraceae, and Euphorbiaceae with 3 species each.Connaraceae, Convolvulaceae, Malvaceae, Meliaceae, and Myrtaceae are represented by 2 species each, whereas 24 families contributed 1 species each (Figure 2).The high occurrence of the family Fabaceae could be explained by the fact that most species belonging to the family Fabaceae are mostly found throughout the seasons because they are adapted to withstand the adverse effects of Sahel regions as reported by Kankara et al. (2015).This is however, contrary to the findings of Kalaskar and Surana (2004) who reported that majority of plants used against liver diseases by different tribes of India belong to the Malvaceae family.While majority of plants used to treat liver problems in Maritime region of Togo belong to Caesalpiniaceae (Kpodar et al., 2015).Majority of the plants used to treat liver diseases in the study area (38.71%) were reported as trees (Figure 3), followed by shrubs (37.10%), and herbs (24.19%).This may be due to the fact that this growth form is available in almost all seasons and in addition are not affected by seasonal variations as reported by Albuquerque (2006).Majority (79.03%) of the plants reported in this study are sourced from the wild (Figure 4).This may not be unconnected to the belief that wild plants have more healing power than their cultivated counterparts.Similar findings were also reported from Togo and India (Haile and Delenasaw, 2007;Kpodar et al., 2015).This has a negative consequence on the plants' diversity of the area as the area is already being faced by other ecological problems.Leaves are the main used plant part in this study (45.16%), followed by the bark (35.48%), and the whole plant (24.19%), whereas the fruit, flower, rhizome, root, stem, and seed account only for 16.11% all together.This corresponds with the findings of other ethnomedicine studies in Africa like Uganda, Ethiopia and Mali where it was reported that most of the plant parts used in different preparations for remedy were the leaves (Tagola, and Diallo 2005).Most of the medications (80.65%) are prepared as decoction (Figure 5), then powder (16.13%), and maceration (3.23%).This also agrees with the findings of Kpodar et al. (2016) who found that medicinal plants used for liver diseases in the Maritime Region of Togo are mostly prepared as decoctions.
White potassium is added in most cases to the      decoctions to inactivate the bitter taste of the medications.Various additives such as, cow milk, porridge, honey, etc were mixed with the powdered medicines in remedy preparations.More than one plant species have been reported to be used by the people in remedy preparation for hepatic ailments.This could be attributed to additives or synergistic effect that they could have during treatment (Haile and Delenasaw, 2007), while some plants are prepared singly and this agrees with other findings in Bolivia (Macia, 2005).Following the interview with traditional healers it has been reported that majority were found to have poor knowledge of dosage and antidote while giving prescription of remedy to the patients, and most of the preparations were said to have no side effect except vomiting and in rare cases watery stool and this may be attributed to the low toxicity of medicinal plant species used by the local herbalist (Haile and Delenasaw, 2007).
The major threat to the availability of medicinal plants in the study area was deforestation.This could be attributed to the additional values of the majority of ethnomedicinal plants in the study area as well as current high demand for fuel wood as an energy source.Therefore effort should be made to conserve the diversity of these vital resources.

Conclusion
This study provides the first ethnobotanical data on the use of plants to manage hepatic ailments in the study area.From the study, it is evident that people in the study area still rely on medicinal plants for their primary healthcare.There is no doubt that this study will greatly help in preventing the erosion of indigenous knowledge.Considering the fact that most of the plants reported in this study appeared to be rare, there is an urgent need for strategies towards conserving such vital resources.Further researches aimed at validating the folkloric use of the surveyed plants is also ideal.

Figure 1 .
Figure 1.Map of Katsina State, Nigeria showing the study area.

Figure 2 .
Figure 2. Distribution of plant families used for the management of hepatic ailments in Katsina State, Nigeria.

Figure 3 .
Figure 3. Habit status of medicinal plants used for the management of hepatic ailments in Katsina State, Nigeria.

Figure 4 .
Figure 4. Habitat status of the medicinal plants used for the management of hepatic ailments in Katsina State, Nigeria.

Fig. 2 :
Fig. 2: Distribution of plant families used for the management of hepatic ailments in Katsina State, Nigeria.
The state is bounded by Niger Republic to the north, to the east by Jigawa and Kano States, Kaduna State to the south and Zamfara State to the west.The state has 34 local government areas which are categorized into three Senatorial Zones, namely the Katsina South, Katsina North, and the Katsina Central Senatorial Zones.From each Senatorial Zones, one local government was selected for the purpose of this research.

Table 1 .
Socio-demographic information of the respondents of ethnobanical survey of medicinal plants used for the management of hepatic ailments in Katsina State, Nigeria.