This study evaluated the phytoconstituents of the chloroform extract of Chasmenthera dependens (C. dependens) roots and the effect of the extract on the liver serum/homogenate enzyme activities of carbon Tetrachloride Induced hepatoxicity in Wistar Albino Rats. Twenty-four (24) female albino rats were used for this study and were divided into groups of four rats each: Groups I rats were normal control. Hepatotoxicity was induced with the use of carbon tetrachloride on Groups II, III, IV, V and VI. Group II was left untreated and served as the experimental control while Groups III, IV and V were treated with extract, administered orally at three dose level (200, 400 and 600mg/kg body weight of the extract respectively) while group VI was treated with 200mg/kg body weight of Stalvan (standard drug). Phytochemical analyses, activities of serum and homogenate Alanine transaminase (ALT), Aspartate transaminase (AST), Alkaline phosphatase (ALP) and total bilirubin (T.Bil) were determined using standard methods. Phytochemical analysis revealed the presence of phenolics, flavonoids, terpenoids, steroids, alkaloids, tannnins, carbohydrate and reducing sugar. There was generally a significant (p<0.05) dose-dependent decrease liver enzyme (AST, ALP and ALT) and total bilirubin level of Groups III, IV and V as compared to Group II that was left untreated. Although Group VI highly decreased the activities of liver enzymes and total Bilirubin, it is noteworthy that most increases in the dosage of the extracts in Group V superseded those of Group VI. The result of this study suggests that the chloroform extract of Chasmenthera dependens roots contains some bioactive compounds that possess strong hepato-protective as well as hepato-curative effect on CCl4 induced oxidative damage.