June 2012
Phytochemistry and pharmacological properties of Ruta graveolens L.
Ruta graveolens is known as Rue. R. graveolens extracts and essential oil are important areas in drug development with numerous pharmacological activities in many countries. For a long time R. graveolens has been used in traditional medicines for the relief of pain, eye problems, rheumatism and dermatitis. R. graveolens has recently been shown to have antibacterial, analgesic,...
June 2012
Biological activities and anticorrosion efficiency of water hyacinth(Eichhornia crassipes)
Eichhornia crassipes (Mart) solms is an invasive macrophyte, causing serious problems to the network of irrigation and drainage canals in the Nile Delta region and different places around the world. Plants and hydrophytes (as water hyacinth) have increasingly been shown to provide rich source of natural bioactive compounds with antimicrobial, antitumoral, antiviral, and antioxidant...
June 2012
Optimization for ultrahigh pressure extraction of berberine from Cortex Phellodendri by central composite design-response surface methodology
As a novel extraction technique, ultrahigh pressure extraction (UPE) was used to extract berberine from Cortex Phellodendri firstly, which procedure has higher extraction yield, lower extracting temperature, shorter extracting time and less power consumption. The content of berberine was determined by reverse phase-high performance liquid chromatography (RP-HPLC). The UPE process of berberine from Cortex...
June 2012
Antioxidant and anti-inflammatory compounds isolated from Acer tegmentosum
The chromatographic separation of methanol extract from Acer tegmentosum twigs led to the isolation of one phytosterol and five phenolic compounds. Using spectroscopic methods, the structures of these compounds were determined as β-sitosterol 3-O-β-D-glucopyranoside (1), ρ-hydroxyphenylethyl-O-β-D-glucopyranoside (Salidroside) (2), ρ-hydroxyphenylethyl alcohol (tyrosol)...
June 2012
In vitro Leishmanicidal activity of Calotropis gigantea and its fractions against Leishmania major
Cutaneous leishmaniasis (CL) which is caused by the different species of Leishmaina, produces a skin ulcer that heals spontaneously in most cases, leaving an unsightly scar. In the traditional medicine, the extracts of different species of Calotropis gigantea have been successfully used for the treatment of cutaneous leishmaniasis in folklore medicine in south east of Iran. There has been no scientific...
June 2012
Analysis of genetic diversity in Hedychium spicatum Buch. Ham. ex. D. Don in West Himalaya, India
Molecular profiling of 12 different accessions of Hedychium spicatum were investigated using 25 random amplification of polymorphic DNA (RAPD) primers. A total of 240 amplification products were scored with an average frequency of 14.11 bands per primer.Based on the Jaccard's similarity coefficient, 12 accessions were grouped into two main clusters with 38% similarity. The data...
June 2012
Hepatocyte growth factor-loaded ultrasound contrast agent for restoration of injured facial nerves in Sprague Dawley (SD) rats
The purpose of the study was to explore the regeneration effect of the ultrasound-targeted microbubble destruction-mediated hepatocyte growth factor (HGF) gene system on facial nerve injury in rats. Forty (40) rats were randomly divided into 4 groups after the models of facial nerve injury were established: A, phosphate buffered saline (PBS) group; B, HGF and microbubble (HGF + MB) group; C, HGF and ultrasound (HGF +...
June 2012
In vitro selection for resistance to Fusarium oxysporum f. sp. dianthi and detection of genetic polymorphism via RAPD analysis in carnation
Embryogenic calli derived from leaf base explants of two carnation cultivars, Lia and White Liberty, were cultured in vitro for resistance to toxic metabolites produced by Fusarium oxysporum f.sp.dianthi, using two selection methods, the double – layer culture and culture filtrate techniques. Results indicated that the reduction of callus growth rate was higher with the in...
June 2012
Phytochemical analysis and biological activities of selected medicinal plants
The antibacterial and antifungal activities of water and chloroform extracts of Acorus calamus, Aremisia annua, Chenopodium foloisom, Euphobia heliscopia and Cupressus sempervirens were carried out against six bacterial strains Bacillus subtillis, Proteus vulgaris, Staphylococus aureus (Gram-positive), Escherichia coli, Pseudomonas aeruginosa, Salmonella...
June 2012
Effect of plant growth regulators on high frequency in vitro multiplication of a vulnerable woody medicinal climber Embelia ribes Burm. f.
A viable high frequency micro-cloning protocol was standardized from nodal explants of 25 years old Embelia ribes cultured in Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of plant growth regulators (PGRs). Among the PGRs tested, MS medium supplemented with 0.5 mg/L BA was found to be most effective for shoot bud induction resulting in...
June 2012
Determination of protopine in rat brain after oral administration of the extract of Corydalis decumbentis
A simple and sensitive high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed to determine protopine in rat brain samples after oral administration of the extract of Corydalis decumbentis. The extract of C. decumbentis was administered at a dose of 6.88 g/kg (equivalent to 159.62 mg/kg of protopine). The lower limit of quantification was 18.9 μg/ml for...
June 2012
Genetic erosion risk by environmental and antropic factor applied on strategies for Lychnophora ericoides conservation
This study evaluated the genetic erosion risk factors and the strategic points for the conservation of Lychnophora ericoides population in “Paraíso Perdido” farm, Serra da Canastra (20° 37’ 54” S; 46° 19’ 37” W; 833 m height) in São João Batista do Glória City, Minas Gerais State, Brazil. The number of young and adult plants, the soil and...
June 2012
Evaluation of in vitro antioxidant activity of leaf extract of Alpinia malaccensis
Many oxidative stress related diseases like cancer, diabetes, neurodegenerative disorders and cardiovascular diseases are occurring as a result of accumulation of free radicals in the body. Lots of researches are going on worldwide towards finding natural antioxidants of plant source. In the present study, the antioxidant activity of methanol extract of Alpinia malaccensis leaf was investigated using...
June 2012
Antibacterial potential of plants traditionally used for curing diarrhea in Khyber Pakhtunkhwa, Pakistan
Thirty-three plant species belonging to 26 families are traditionally used for curing diarrheal diseases in Khyber Pukhtunkhwa, Pakistan. The ethanolic extracts of these medicinal plants were screened for their antibacterial potential against five bacterial species. These tests were carried out using agar well diffusion method at three elevating concentrations (10, 20 and 30 mg/ml) of the crude extracts. The results...
June 2012
In vitro antifungal activities of essential oil from Nigerian medicinal plants against toxigenic Aspergillus flavus
The aim of this study is to determine the sensitivity profile of toxigenic mould strain isolated from poultry feed to some essential oils. Essential oils extracted from eight medicinal plants collected from South-West Nigeria were screened for their activity against toxigenicAspergillus flavus by disc diffusion method. Minimum inhibitory concentrations (MICs) of oils (%v/v) against A. flavus done by...
June 2012
Ethylacetate extract of Aquilaria crassna preserve actin cytoskeleton on simulated ischemia induced cardiac cell death
This paper investigates the effect of ethylacetate extract of Aquilaria crassna Pierre ex Lecomte on simulated ischemia-induced cytoskeleton disorganization in cardiac myoblast cell line, so as to provide some evidence for its traditional use. Rat cardiac myoblast cell line (H9c2), were subjected to 2 h simulated ischemia and 24 h of reperfusion, in the presence and absence of the ethylacetate extract...
June 2012
Cytotoxic and anti-inflammatory activity of some Thai medicinal plants
In order to search for new anti-inflammatory agent for use as an adjunctive treatment for periodontal diseases, we have studied crude extracts from plants of family Rutaceae, Asteraceae and Maliaceae which have been claimed to possess anti-microbial and anti-inflammatory activities. The cytotoxic effect of these plant crude extracts on human gingival fibroblasts (HGFs) and monocytes was...
June 2012
Acemannan induces cementoblast proliferation, differentiation, extracellular matrix secretion, and mineral deposition
Acemannan, a polysaccharide extract from Aloe vera gel, has been reported to induce oral wound healing and dentin regeneration in vitro and in vivo. However, the regenerative effect of acemannan on cementoblasts, cementum forming cells, has not been defined. In this study, we hypothesized acemannan would induce cementoblast proliferation, differentiation, extracellular matrix and growth...
June 2012
Antioxidant and anti-inflammatory activities of hot water extract from Pluchea indica Less. herbal tea
In this study, antioxidant activity of the hot water extract of Pluchea indica tea leaves (HWEP) was measured by assays for radical scavenging against 1,1-diphenyl-2-picrylhydrazyl, superoxide and hydroxyl radical, ferric ion reducing power, as well as ferrous ion chelating. Results showed that HWEP exhibited good antioxidant activity in all test systems in a concentration-dependent manner. Furthermore, HWEP...
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