February 2012
An evaluation of the anticancer activity in Hopea odorata extracts
Possible anticancer characteristics of Hopea Odorata extracts were investigated by cell proliferation and viability studies of cells in culture. The mechanism of action was studied by determining the rate of apoptosis and expression of protein involved in signal transduction. The result indicated that the butanol extract of H. Odorata had selective inhibition to both Hep G2 and Chang cells with...
February 2012
Anti-Helicobacter pylori and anti-internalization activities of Thai folk remedies used to treat gastric ailments
Invasion of gastric epithelium cells by Helicobacter pylori is associated with antibiotic resistance and persistence infection, which contribute to eradication failure in many circumstances. The aims of this study were to investigate the anti-H. pylori and anti-internalization activities of thirteen Thai plant extracts used for gastric ailments in traditional medicine including Curcuma...
February 2012
Establishment of plant regeneration of Michelia champaca L. through cell suspension culture technique
Michelia champaca L. is a woody tree species, which has high economical value as a basic material for medicinal and fragrance products. However, there are several problems encountered in conventional propagation of M. champaca, which are generally low through seed germination, while vegetative layering technique produces limited planting materials. This experiment was conducted to establish high...
February 2012
Phytotoxic characterization of various fractions of Launaea nudicaulis
Launaea nudicaulis is a local medicinal plant of District Bannu, KPK, Pakistan, used traditionally for various diseases and having the phytotoxic ability. In the present study, allelopathic activity of the various fractions of Launaea nudicaulis was studied against radish growth. Two concentrations of the extract: 100 and 1000 ppm were used. Methanolic and ethyl acetate fraction markedly showed inhibition...
February 2012
Antioxidant effect of Zanthoxylum limonella Alston.
Zanthoxylum limonella Alston. (Rutaceae) has been widely used as spice and folk medicine. Different part of this plant, such as ripe fruit was used as a condiment in curries, and its essential oil is quite useful for blood circulation and gastrointestinal tract. The bark was noted for its febrifugal, sudorific and diuretic properties. It has been used for treatment of fever caused by free radical production in the...
February 2012
Rapid method for simultaneous determination of 11 chemical constituents in the traditional Chinese medicinal prescription Wu-Ji-San by reverse phase high-performance liquid chromatography coupled with diode array detection (RP-HPLC-DAD)
In the present paper, a simple and sensitive high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method was investigated for simultaneous determination of 11 components in Wu-Ji-San (WJS). The 11 identified components were ephedrine hydrochloride, paeoniflorin, liquiritin, ferulic acid, naringin, hesperidin, cinnamaldehyde, imperatorin, glycyrrhizic acid, honokiol and...
February 2012
Some biochemical parameters and histopathological features following prolonged administration of aqueous pod extract of Acacia nilotica in albino rats
The effects of aqueous pod extract of Acacia nilotica was investigated in rats. The pod extract of A. nilotica was obtained by Soxhlet extraction using distilled water as a solvent. The aqueous extract was administered for a period of 21 days at 50, 100 and 200 and 400 mg/kg body weight, respectively. The liver enzymes and some biochemical parameters were evaluated. The tissue samples were also taken...
February 2012
Kenaf seed oil from supercritical carbon dioxide fluid extraction inhibits the proliferation of WEHI-3B leukemia cells in vivo
Hibiscus cannabinus (kenaf) seed oil is a rich source of bioactive phytochemicals with high anti-oxidative and cancer chemopreventive properties. However, the seeds are disposed aswaste material during the harvesting or processing of kenaf. Preliminary study revealed that kenaf seed oil from supercritical carbon dioxide fluid extraction (SFE) induced apoptosis in WEHI-3B leukemia cells. Thus, this...
February 2012
Limonene synthase gene expression under different concentrations of manganese in Cuminum cyminum L.
SQ-RT-PCR was applied to study Limonene synthase (LS) gene expression pattern and response to different concentrations of manganese in different organs of cumin (Cuminum cyminum L.). The results revealed that the gene is expressed in very small (< 2 mm) and small (3 to 4 mm) flowers and also in shoots, while it is not expressed in roots, leaves, medium (4 to 5 mm) and larger flowers. The highest level of gene...
February 2012
Differences in essential oil content and chemical composition of new Ocimum basilicum genotyps in relation to some quantitative characters
The quantitative characters, content and chemical composition of the essential oil have been evaluated in eight genotypes by hybrid origin of Ocimum basilicum L. that differ also by the color of leaves and flowers. The content of the essential oil in the assessed genotypes vary from 0.403 to 1.405% (dry matter). The values of the essential oil content are more elevated in the majority of the genotypes with red...
February 2012
Morphology and histochemistry of the glandular trichomes of Isodon rubescens (Hemsley) H. Hara [Lamiaceae]: A promising medicinal plant of China
Isodon rubescens, a perennial herb indigenous to China, with medicinal application, potentially has economic value. The morphology of the glandular trichomes was investigated with light microscopy. At the same time the chemical content was analyzed by applying chemical reagents and fluorescence microscopy. This morphoanatomical and histochemical study revealed that leaves of I. rubescens possess one type of...
February 2012
Evaluation of the antioxidant activities of total flavonoids from tartary buckwheat
In this study, the antioxidant activities of total flavonoids from tartary buckwheat (FTB) were evaluated. In vitro, antioxidant activities of FTB were conducted by determining the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and butylated hydroxyl toluene (BHT) radical scavenging activities. In vivo, antioxidant activities of FTB were conducted by determining antioxidant enzymatic activities and malondialdehyde...
February 2012
Antioxidant activities and RP-HPLC identification of polyphenols in the ethyl acetate extract of Tunisian Juglans regia L treated barks.
The barks and leaves of Juglans regia L. are widely used in Tunisia to maintain good oral hygiene and are known to possess high therapeutic effects. In this paper, and for the first time, we report the reversed phase-high performance liquid chromatography (RP-HPLC) identification of polyphenols in the ethyl acetate extract obtained from the barks of J. regia L.and we tested...
February 2012
Bioactivity of the acetone extract of the stem bark of Combretum molle on selected bacterial pathogens: Preliminary phytochemical screening
The antimicrobial activity of fractionated acetone extracts of the stem bark of Combretum molle was evaluated against Helicobacter pylori PE 252C, Streptococcus pyogenes ATCC 49399, Pseudomonas aeruginosa ATCC 15442 and Plesiomonas shigelloides ATCC 51903 in a bid to identify the active constituents of this plant. Fractionation of the acetone extract was done...
February 2012
Influence of medium composition on multiplication of walnut (Juglans regia L.) growth
In vitro proliferation of walnut (Juglans regia var. zeiabadi) was studied in response to the four levels (1/3X, 1/2X, 1.0X and 1.5X) of macrominerals of DKW (Driver and kunjyukj, 1984) medium. Macrominerals (N, P, K and Mg) can be a limiting factor for growth quantity (fresh weight and dry weight) and growth quality (explants appearance) of walnut explants. Addition of 0.5X macrominerals to the basal...
February 2012
Inhibition effects on Hepatitis B virus replication by hydrophobic extracts from Ferula ferulaeoides (Steud.) Korov
Hepatitis B virus (HBV) infection is a worldwide public health problem, which can lead to life threatening chronic hepatitis, liver cirrhosis and cancer. Despite this, treatments for chronic HBV infection are still very limited. In our investigation of herbal medicines as antiviral agents, FF, the lipid soluble fraction of Ferula ferulaeoides, which has been used as a traditional Chinese medicine, showed a...
February 2012
Colchicum autumnale: A review
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