For establishment of the role of peptide Tau and Secretagogin in the oncogenes and tumor-suppressor genes balanced functional activity, malignant RIN-5F cells from rat insulinoma, containing additional copy of the Secretagogin gene, inserted by their transfection with recombinant gene construct pGEX-1Î»T (Amersham Pharmacia Biotech) of Escherichia coli, are used. These cells are
in vitro-co-cultivated with myeloid cell precursors, derived from laboratory-cultivated mouse embryonic stem cells in the presence of Doxyciclin (2 Î¼g/ml - Sigma-Aldrich), probably by the known from literature sources activation of tumor-suppressor genes from STAT-family. According to other literature findings, a calcium-dependent SCGNâ€“TAU interaction, as well as co-appearance of both proteins was shown. Furthermore, the so induced Secretagogin over-expression could exert protective function on the transfected Rin-5F cells. These data could be confirmed by noticed by us differences in the degree of further myeloid differentiation of the derived precursor cells in their in vitro-co-cultivation with transfected with additional copy of the Secretagogin gene Rin-5F malignant rat insulinoma cells, in comparison with the results, obtained in their laboratory co-cultivation with human cervical carcinoma Hela cells.
Keywords: : neuropeptides, oncogenes, tumor-suppressor genes.