Journal of
Neuroscience and Behavioral Health

  • Abbreviation: J. Neurosci. Behav. Health
  • Language: English
  • ISSN: 2141-2286
  • DOI: 10.5897/JNBH
  • Start Year: 2009
  • Published Articles: 65

Article in Press

Role of Peptides Secretagogin and Tau in Oncogenes and Tumor-Suppressor Genes Balanced Functional Activity

Iskra Sainova1, Ilina Vavrek1, Velichka Pavlova1, Theodora Daneva2, Elena Nikolova1

  •  Received: 20 June 2011
  •  Accepted: 09 November 2011
For establishment of the role of peptide Tau and Secretagogin in the oncogenes and tumor-suppressor genes balanced functional activity, malignant RIN-5F cells from rat insulinoma, containing additional copy of the Secretagogin gene, inserted by their transfection with recombinant gene construct pGEX-1λT (Amersham Pharmacia Biotech) of Escherichia coli, are used. These cells are in vitro-co-cultivated with myeloid cell precursors, derived from laboratory-cultivated mouse embryonic stem cells in the presence of Doxyciclin (2 μg/ml - Sigma-Aldrich), probably by the known from literature sources activation of tumor-suppressor genes from STAT-family. According to other literature findings, a calcium-dependent SCGN–TAU interaction, as well as co-appearance of both proteins was shown. Furthermore, the so induced Secretagogin over-expression could exert protective function on the transfected Rin-5F cells. These data could be confirmed by noticed by us differences in the degree of further myeloid differentiation of the derived precursor cells in their in vitro-co-cultivation with transfected with additional copy of the Secretagogin gene Rin-5F malignant rat insulinoma cells, in comparison with the results, obtained in their laboratory co-cultivation with human cervical carcinoma Hela cells.

Keywords: : neuropeptides, oncogenes, tumor-suppressor genes.