Journal of
Physiology and Pathophysiology

  • Abbreviation: J. Physiol. Pathophysiol.
  • Language: English
  • ISSN: 2141-260X
  • DOI: 10.5897/JPAP
  • Start Year: 2010
  • Published Articles: 43

Full Length Research Paper

Preliminary study of physiopathological changes associated with the ethanolic extract of the stem leaves of Phyllanthus amarus in rats

MAHAMADOU DANDA Amina
  • MAHAMADOU DANDA Amina
  • Laboratoire de Physiopathologie Moléculaire et Toxicologie, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-calavi 01 BP: 4521 Cotonou, Benin.
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ATTAKPA Sèlidji Eugène
  • ATTAKPA Sèlidji Eugène
  • Laboratoire de Physiopathologie Moléculaire et Toxicologie, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-calavi 01 BP: 4521 Cotonou, Benin.
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BÉHANZIN Gbèssohélè Justin
  • BÉHANZIN Gbèssohélè Justin
  • Laboratoire de Physiopathologie Moléculaire et Toxicologie, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-calavi 01 BP: 4521 Cotonou, Benin.
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AMOUSSA Abdou Madjid
  • AMOUSSA Abdou Madjid
  • Unité de Biochimie et de Biologie Moléculaire, Laboratory of Biochemistry and Bioactives, Natural Substances, Faculty of Science and Technology, University of Abomey-calavi 01 BP: 4521 Cotonou, Benin.
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LAGNIKA Latifou
  • LAGNIKA Latifou
  • Unité de Biochimie et de Biologie Moléculaire, Laboratory of Biochemistry and Bioactives, Natural Substances, Faculty of Science and Technology, University of Abomey-calavi 01 BP: 4521 Cotonou, Benin.
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AKOTÈGNON Rodrigue
  • AKOTÈGNON Rodrigue
  • Laboratoire de Physiopathologie Moléculaire et Toxicologie, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-calavi 01 BP: 4521 Cotonou, Benin.
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YÉDOMONHAN Hounnankpon
  • YÉDOMONHAN Hounnankpon
  • Laboratoire de Botanique et Ecologie végétal, Département de Biologie Végétale, Faculty of Science and Technology, University of Abomey-calavi 01 BP: 4521 Cotonou, Benin.
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SEZAN Alphonse
  • SEZAN Alphonse
  • Laboratoire de Biomembranes et Signalisation Cellulaire, Department of Animal Physiology, Faculty of Science and Technology, University of Abomey-calavi 01 BP: 4521 Cotonou, Benin.
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BABA-MOUSSA Farid
  • BABA-MOUSSA Farid
  • Laboratoire de Microbiologie et de Technologie Alimentaire, Département de Biologie Végétale, Faculty of Science and Technology, University of Abomey-calavi 01 BP: 4521 Cotonou, Benin.
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BABA- MOUSSA Lamine
  • BABA- MOUSSA Lamine
  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculty of Science and Technology, University of Abomey-calavi 05 BP 1604 Cotonou, Benin.
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  •  Received: 26 February 2018
  •  Accepted: 26 March 2018
  •  Published: 31 March 2018

Abstract

The present work aimed to investigate physiopathological changes associated with the ethanolic extract of the stem leaves of Phyllanthus amarus (EEt(OH) P.a. Exploratory tests are performed in vivo on Wistar albino rats. Analysis of the variation in body weight at the level of the batch having received, and the control group showed a slight weight drop 7 d after treatment. This variation is not significant (p>0.05). The means relative organ weights show that there were no significant changes between control and treated groups (P ˃ 0.05).   No obvious clinical signs (tremor, breathing rate, paralysis). Slightly greater differences were noted for WBC of the Control group and rats treaded with EEt(OH) P.a (5000 mg/kg b.w.) on day 14 after the administration. These differences were statistically significant (p<0.05). Similarly, the platelet count was slightly lower for the control rats compared to the rats treaded. The various biochemical parameters explored, Glucose, Bilirubin (BIL), Alanine  amino transferase (ALT), Aspartate amino transferase (AST), Total protein (TP), alkaline phosphatase (ALP), urea (URE), creatinine (CR), Total bilirubin (TBIL) and Indirect bilirubin (IBIL),  have informed us about the probable effects of the stem leaves of Phyllanthus amarus in the liver and kidney.  There was no significant differences (p>0.05) in glucose, total protein, urea, creatinine levels  between control and experiemental groups at 14 d. Total bilirubin (TBIL) and Indirect bilirubin (IBIL), however, were statistically significant (p<0.05). Other biochemical parameters such as ALT, AST, ALP show statistically insignificant difference (p>0.05) in test and control batch.

Key words: Phyllanthus amarus Schum. & Thonn., physiopathological, breathing rate, paralysis, bilirubin, alanine amino, transferase, creatinine, alkaline, phosphatase, transaminases, aspartate amino.

Abbreviation

EEt(OH)P.a,  Ethanolic extract of the stem leaves of Phyllanthus amarus Schumach. & Thonn.; WBC, white blood cells; AST, aspartate amino transferase; ALT, Alanine amino transferase; ALP, alkaline phosphatase; BIL, bilirubin; TBIL, total bilirubin; IBIL, indirect bilirubin; TP, total protein; CR, creatinine; URE, urea.