Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 409

Review

Current progress of biosafe selectable markers in plant transformation

Zhengyi Wei1, Xingzhi Wang2 and Shaochen Xing2,1*
  1Biotechnology Research Centre, Jilin Academy of Agricultural Sciences, 1363, Caiyu Street, Changchun, 130033, People’s Republic of China. 2School of Life Sciences, Northeast Normal University, 5268, Renmin Street, Changchun, 130024, People’s Republic of China
Email: [email protected]

  •  Accepted: 04 January 2012
  •  Published: 15 January 2012

Abstract

 

Plant transformation plays a crucial role in modern plant breeding system, leading to the improvement of agronomical traits and fundamental studies of plant science. To develop an efficient and reliable transformation approach, selectable marker is the prerequisite and extremely useful in enabling successful transformation. A number of environmental safe and user-friendly selectable marker genes have been exploited in recent years and utilized to mitigate as a result of concerns for bio-security. Considering the different function of each of the marker genes, these are generally divided into four groups namely positive, visual, negative and pathogen-based, respectively, depending on their selection mechanism. This review describes the mechanism of each of these categories of selected markers and summarizes the current progress in genetic transformation, helping researchers to make better judgement on the choice of selectable markers and accelerate their potential application during plant genetic transformation.

 

Key words: Bio-safety, selectable marker, plant transformation.