Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 416

Full Length Research Paper

Effectiveness of pyramided genes in conferring resistance to anthracnose disease in common bean populations

Kiryowa M.
  • Kiryowa M.
  • Department of Agricultural Production, College of Agricultural and Environmental Sciences, Makerere University, P. O. Box 7062, Kampala, Uganda.
  • Google Scholar
Nkalubo S. T.
  • Nkalubo S. T.
  • National Crops Resources Research Institute (NaCRRI), NARO, P. O. Box 7064, Kampala, Uganda.
  • Google Scholar
Mukankusi C.
  • Mukankusi C.
  • Centro Internacional de Agricultura Tropical (CIAT), P. O. Box 6247, Kampala, Uganda.
  • Google Scholar
Male A
  • Male A
  • Centro Internacional de Agricultura Tropical (CIAT), P. O. Box 6247, Kampala, Uganda.
  • Google Scholar
Gibson P.
  • Gibson P.
  • Department of Plants, Soils, Agricultural Systems, Southern Illinois University, Carbondale, IL, USA.
  • Google Scholar
Tukamuhabwa P.
  • Tukamuhabwa P.
  • Department of Agricultural Production, College of Agricultural and Environmental Sciences, Makerere University, P. O. Box 7062, Kampala, Uganda.
  • Google Scholar
Rubaihayo P.
  • Rubaihayo P.
  • Department of Agricultural Production, College of Agricultural and Environmental Sciences, Makerere University, P. O. Box 7062, Kampala, Uganda.
  • Google Scholar


  •  Received: 01 March 2019
  •  Accepted: 01 May 2020
  •  Published: 31 January 2021

Abstract

Anthracnose disease (Colletotrichum lindemuthianum (Sacc. et. Magn) Lams. Scrib.) is one of the most devastating diseases that constrain common bean production in Uganda. A cascading pedigree pyramiding scheme was used to develop common bean populations to evaluate the effectiveness of pyramided and single resistance genes (Co-42, Co-43, Co-5, and Co-9) on disease development. Detached leaf trifoliates of F4:6 genotypes were screened with four C. lindemuthianum races (352, 713, 767 and 2047). Disease severity data were subjected to ANOVA. Races, genotypes and Race x Genotype interaction were significant. Genes Co-42 and Co-5 conferred resistance to the four races and the gene pyramids Co-42+Co-5+Co-9 and Co-42+Co-5 had the lowest severity scores. Gene Co-43 conferred resistance to race 352 and weak resistance to race 713; whereas, gene Co-9 conferred resistance to race 352.  Co-43+Co-9 gene pyramid showed resistance only to race 352. The Co-42 and Co-5 genes conferred resistance to all the four races 352, 713, 767 and 2047. The single gene Co-42 was not significantly different from the pyramids Co-42+Co-5+Co-9 and Co-42+Co-5 (P<0.01). Similarly, the Co-5 gene was not significantly different from Co-42+Co-5, Co-42+Co-9 and Co-5+Co-9 pyramids. The Co-9 gene showed antagonism in all pyramids. These results indicate that pyramiding of resistance genes would be effective for disease management in Uganda, but pyramids with Co-9 gene would be less effective.

Key words: SCAR markers, Colletotrichum lindemuthianum, broad-spectrum resistance.