Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 409

Full Length Research Paper

Application of marker-assisted backcrossing to improve cowpea (Vignaunguiculata L. Walp) for drought tolerance

Benoit Joseph Batieno*
  • Benoit Joseph Batieno*
  • Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou, Burkina Faso.
  • Google Scholar
Eric Danquah
  • Eric Danquah
  • West Africa Centre for Crop Improvement/ University of Ghana (WACCI/UG), Ghana.
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Jean-Baptiste Tignegre
  • Jean-Baptiste Tignegre
  • World Vegetable Centre (AVRDC) Shanhua, Tainan, Taiwan.
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Bao-Lam Huynh
  • Bao-Lam Huynh
  • University of California Riverside (UCR), USA.
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Issa Drabo
  • Issa Drabo
  • Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou, Burkina Faso.
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Timothy J Close
  • Timothy J Close
  • University of California Riverside (UCR), USA.
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Kwadwo Ofori
  • Kwadwo Ofori
  • West Africa Centre for Crop Improvement/ University of Ghana (WACCI/UG), Ghana.
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Philip Roberts
  • Philip Roberts
  • University of California Riverside (UCR), USA.
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Tinga Jeremy Ouedraogo
  • Tinga Jeremy Ouedraogo
  • Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou, Burkina Faso.
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  •  Received: 31 August 2016
  •  Accepted: 17 October 2016
  •  Published: 31 December 2016

Abstract

Molecular-assisted backcrossing (MABC) was used to introgress drought tolerance, Striga and root-knot nematode resistance QTLs into a farmer-preferred widely grown cowpea landrace adapted for intercropping in Burkina Faso. Two backcross populations were developed using two drought tolerant donor lines IT93K-503-1 (nematode resistant) and IT97K-499-35 (Striga resistant) and the drought sensitive landrace Moussa Local as the recurrent parent.  A set of 184 genomewide EST-derived SNP markers spanning an average of 2-cM intervals and flanking known trait positions was employed for genotyping the backcross progenies using the cowpea KASP genotyping platform. BC1F1 individual plants that were heterozygous for SNPs associated with drought tolerance, Striga and/or nematode resistance (foreground SNPs) and carried as many recurrent-parent alleles as possible at other SNP loci (background SNPs) were selected for the next backcross cycle. This process was repeated to produce BC3F1 families of each donor population. The six best families from the two donors based on marker aided selection and preliminary yield performance under well-watered and water-restricted field trials and Striga resistance screening were selfed to increase seed (BC3F2) for further yield tests. This study demonstrated the high efficiency of using SNP markers in foreground and background marker selection in a MABC scheme to improve a widely grown cowpea variety by adding drought tolerance and biotic stress resistance traits.

Key words: Molecular-assisted backcrossing (MABC), drought tolerance, Striga, root-knot nematode, QTLs, EST-derived SNP markers, cowpea.