Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 409

Full Length Research Paper

Identification of coupling- and repulsion-phase markers in rice for brown planthopper resistance genes using F2S of IR 50 X CO 46

  Venkateswarlu Yadavalli1,2*, Gajendra P. Narwane2, P. Nagarajan2 and M. Bharathi3   
  1Department of Biochemistry, School of Life Sciences, University of Hyderabad. Hyderabad (AP), India-500 046, India.    2Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu,  India-641003, India.    3Department of Entomology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu, India-641003, India. 
Email: [email protected], [email protected]

  •  Published: 31 January 2011

Abstract

 

Brown planthopper, Nilaparvata lugens Stål (Homoptera: Delphacidae) is one of the most threatening 
pests, often significantly reducing the rice yield. The breeding of resistant cultivar has been the most 
effective way of controlling this pest. Recent advancements in DNA marker technology together with 
the concept of marker-assisted selection (MAS) provide new solutions for selection of more durable 
brown planthopper (BPH) resistant genotypes in rice. F2s of a cross between IR 50 and CO 46 and their 
susceptible and resistant parents were used for the present study. In bulk segregant analysis, random 
amplification of polymorphic DNA (RAPD) primer, OPC 11 (5’AAAGCTGCGG 3’) showed co-dominant 
banding pattern, which amplified a susceptible phenotype specific marker OPC11856 associated with 
repulsion phase. 
It also amplified resistant phenotype specific markers,
 OPC 11817 which are associated 
in coupling phase to the resistant allele. The OPA11817 RAPD marker could be used in a cost effective 
way for marker assisted selection of BPH resistant rice genotypes.
 
 
Key words: RAPD markers, brown planthopper, bulk segregant analysis, hopper burn, Oryza sativa.