Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 409

Full Length Research Paper

Genetic diversity analysis in different varieties of black gram using RAPD markers

Priya Srivastava1, Anjana Pandey1* and Diamond Prakash Sinha2
  1Nanotechnology and Molecular Biology Lab, Centre for Biotechnology, University of Allahabad, Allahabad, 211002, U.P., India. 2Indian Institutes of Vegetable Researches (ICAR), Jakhani, Varanasi, U.P. 221002, India.
Email: [email protected]

  •  Accepted: 01 March 2011
  •  Published: 31 March 2011

Abstract

 

The molecular marker is a useful tool for assessing genetic variation and resolving cultivars identities. The objective of the present study was to investigate the genetic diversity and relationships among 8 black gram (Vigna mungo) varieties from diverse geographic locations of U.P. and Andhra Pradesh, (India) using random amplified polymorphic deoxyribonucleic acid (RAPD) markers. Forty decamer - primers could generate a total of 346 RAPD fragments, of which 338 or 97.68% were polymorphic. The size of amplified fragments was ranged from 50 to 3000 bp. The similarity coefficient was maximum between PLU-289 and PLU-456 (0.76) indicated the less divergence between them. Lower similarity was observed between LBG-20 and PLU-289 (0.4337) indicating more divergence.

 

Key words: Black gram, random amplified polymorphic deoxyribonucleic acid, genetic diversity, similarity coefficient, polymorphism.