The molecular marker is a useful tool for assessing genetic variation and resolving cultivars identities. The objective of the present study was to investigate the genetic diversity and relationships among 8 black gram (Vigna mungo) varieties from diverse geographic locations of U.P. and Andhra Pradesh, (India) using random amplified polymorphic deoxyribonucleic acid (RAPD) markers. Forty decamer - primers could generate a total of 346 RAPD fragments, of which 338 or 97.68% were polymorphic. The size of amplified fragments was ranged from 50 to 3000 bp. The similarity coefficient was maximum between PLU-289 and PLU-456 (0.76) indicated the less divergence between them. Lower similarity was observed between LBG-20 and PLU-289 (0.4337) indicating more divergence.
Key words: Black gram, random amplified polymorphic deoxyribonucleic acid, genetic diversity, similarity coefficient, polymorphism.
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