Abstract

Medicinal plant extracts are known to possess breast cancer antidote. The present investigation is focused on anticancer efficacy of various parts of Annona squamosa. The organic (ethanol) extracts from various parts of Annona squamosa were prepared using soxhlet apparatus and tested for in vitro anticancer efficacy on Breast cancer cell line MCF-7 by MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results obtained from MTT assay showed that the inhibitory concentration values of bark, peel and seed were found to be approximately 20, 30 and 10 μg/ml, respectively The ethanolic seed extract had high anticancer activity with IC50 value of 10 ug/ml, reveals that A. squamosa inhibits the proliferation of MCF-7 by inducing apoptosis. The plant investigated has anti-cancer activity; hence further studies should be carried out for the isolation of the lead molecules from the parts of the plant to treat the breast cancer.

Key words: Annona squamosa, MCF-7 cell line, 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, anti-cancer activity.