Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 229

Full Length Research Paper

Chemical composition and biological activity of essential oils of Cleopatra mandarin (Citrus reshni) cultivated in Egypt

Dalia I. Hamdan, Rehab H. Abdulla, Maged E. Mohamed and Assem M. El-Shazly*
Pharmacognosy Department, Faculty of Pharmacy, University of Zagazig, Zagazig 44519, Egypt.
Email: [email protected]; [email protected].

  •  Accepted: 18 April 2013
  •  Published: 31 May 2013


A comparative study of the essential oils isolated from the leaf and fruit peel of Cleopatra mandarin (Citrus reshni) was carried out using gas liquid chromatography (GLC) and GLC/mass analysis (MS). Over 140 components were observed in GLC/MS, of which 123 could be identified. 112 compounds were quantitatively analyzed in the leaf oil with linalool as a major component. The total identified components in the fruit peel oil were 69 and limonene was the most prominent. Cleopatra mandarin volatile components showed high anti-inflammatory activity represented by its effect on tumour necrosis factor-α and nitric oxide. They also showed significant anti-microbial activities against most common Gram positive and Gram negative bacteria and some fungi.  


Key words: Cleopatra mandarin, essential oils, gas liquid chromatography (GLC),GLC/mass analysis (MS), anti-inflammatory, antimicrobial.