Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

Bioautographic determination of the antistaphylococcal components of the stem bark of Parkia biglobosa (JACQ) BENTH (MIMOSACEAE)

Udobi Chinweizu Ejikeme1*, Onaolapo Ademola Josiah2 and Abdulsalaam I. A2
1College of Science and Technology, Kaduna Polytechnic, Kaduna, Nigeria. 2Department of Pharmaceutics and Pharmaceutical Microbiology, Ahmadu Bello University, Zaria, Nigeria.
Email: [email protected]

  •  Accepted: 08 October 2010
  •  Published: 31 December 2010

Abstract

Bioautography, a scientific method which combines chromatography with bioassay was used to attempt to determine the bioactive components in the methanolic extract of the stem bark of Parkia biglobosa.Results obtained confirmed 4 compounds of different Rvalues. Each of them showed activity againstStaphylococcus confirming previous results reported and the method also showed the components were acting in synergy. Pseudomonas and Bacillus subtilis were not as susceptible as Staphylococcusto the components. P. biglobosa remains a hopeful source of compounds that can be effectively used against even resistant Staphylococcus.

 

Key words: Bioautography, methanolic, Parkia, Staphylococcus, synergy.