Bioautography, a scientific method which combines chromatography with bioassay was used to attempt to determine the bioactive components in the methanolic extract of the stem bark of Parkia biglobosa.Results obtained confirmed 4 compounds of different Rf values. Each of them showed activity againstStaphylococcus confirming previous results reported and the method also showed the components were acting in synergy. Pseudomonas and Bacillus subtilis were not as susceptible as Staphylococcusto the components. P. biglobosa remains a hopeful source of compounds that can be effectively used against even resistant Staphylococcus.
Key words: Bioautography, methanolic, Parkia, Staphylococcus, synergy.
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