Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 229

Analysis of bioactive chemical compounds of Nigella sativa using gas chromatography-mass spectrometry

Mohammed Yahya Hadi
  • Mohammed Yahya Hadi
  • College of Biotechnology, Al-Qasim Green University, Iraq.
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Ghaidaa Jihadi Mohammed
  • Ghaidaa Jihadi Mohammed
  • College of Science, Al-Qadisia University, Iraq.
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Imad Hadi Hameed*
  • Imad Hadi Hameed*
  • Department of Biology, Babylon University, Iraq.
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  •  Received: 18 August 2015
  •  Accepted: 17 November 2015
  •  Published: 29 February 2016

Abstract

Phytochemicals are chemical compounds often referred to as secondary metabolites. Twenty eight bioactive phytochemical compounds were identified in the methanolic extract of Nigella sativa. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, and molecular formula. Gas chromatography-mass spectrometry (GC-MS) analysis of Nigella sativa revealed the existence of the ß-Pinene, D-Glucose, 6-O-α-Dgalactopyranosyl, O-Cymene, DL-Arabinose, Trans-4-methoxy thujane, 2-Propyl-tetrahydropyran-3-ol, Terpinen-4-ol, α- D-Glucopyranoside, O-α-D-glucopyranosyl-(1.fwdarw.3)-ß-D-fruc, Thymoquinone, 2-Isopropylidene-5-methylhex-4-enal, Limonen-6-ol, pivalate, Longifolene, 2-(4-Nitrobutyryl)cyclooctanone, ß-Bisabolene, 1,1-Diphenyl-4-phenylthiobut-3-en-1-ol, Phenol, 4-methoxy-2,3,6-trimethyl, Pyrrolidin-2-one-3ß-(propanoic acid, methyl ester),5-methylene-4α, Cholestan-3-ol, 2-methylene-,(3ß,5α), l-(+)-Ascorbic acid 2,6-dihexadecanoate, 9,12-Octadecadienoic acid (Z,Z)-, methyl ester, 1-Heptatriacotanol, 10,13-Eicosadienoic acid, methyl ester, E,E,Z-1,3,12-Nonadecatriene-5,14-diol, 9-Octadecenamide,(Z), 2H-Benzo[f]oxireno[2,3-E]benzofuran-8(9H)-one,9-[2-(dimethylar, Phthalic acid, decyl oct-3-yl ester, 1,2-Benzenedicarboxylic acid, bis(8-methylnonyl) ester and Stiqmasterol.

Key words: Gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, Nigella sativa, phytochemicals.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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