Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

Identification of five newly described bioactive chemical compounds in methanolic extract of Mentha viridis by using gas chromatography - mass spectrometry (GC-MS)

Imad Hadi Hameed*
  • Imad Hadi Hameed*
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Hussein J. Hussein
  • Hussein J. Hussein
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Muhanned Abdulhasan Kareem
  • Muhanned Abdulhasan Kareem
  • Babylon University, Centre of Environmental Research, Iraq.
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Nidaa Shihab Hamad
  • Nidaa Shihab Hamad
  • Babylon University, Centre of Environmental Research, Iraq.
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  •  Received: 21 April 2015
  •  Accepted: 09 June 2015
  •  Published: 31 July 2015

Abstract

The bioactive compounds were screened by gas chromatography-mass spectrometry (GC-MS) method. Twenty one bioactive phytochemical compounds were identified in the methanolic extract of Mentha viridis using GC-MS method. The identification of phytochemical compounds is based on the peak area, retention time, molecular weight and molecular formula. GC-MS analysis of M. viridis revealed the existence of the 3,6-Octadecadiynoic acid, methyl ester, 2,5-Dimethyl-4-hydroxy-3(2H)-furanone, 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl, benzofuran, R-Limonene, 2-methoxy-4-vinylphenol, 2-hydroxy-5-methylbenaldehyde, tetra-acetyl-d-xylonic nitrile, Ficusin, Phen-1,4-diol, 2,3-dimethyl-5-trifluoromethyl, n-Hexadecanoic acid, 7-Methyl-Z-tetradecen-1-ol acetate, Ethyl 9,12,15-octadecatrienoate, Methyl 19-methyl-eicosanoate, Ethyl iso-allocholate, and Tocopherol. Five new bioactive chemical compounds 3-(N,N-Dimethyllayrylammonio), 1b,4a-eboxy-2H-cyclopenta [3,4]cyclopropal[8,9] cycloundec, 5H-Cyclopropa[3,4]benz [1,2-e]azulene-5-one, 2,2,4-Trimethyl-3-(3,8), 12,16-tetramethyl-hepta deca, and 4H-Cyclopropa[5,6]benz [1,2:7,8] azulene[5,6-b] oxiren-4-one are described and may in future be suitable sources for phytotherapy purposes. Mentha viridis contain chemical constitutions which may be useful for various herbal formulation exhibiting cardiac tonic, analgesic, antiasthamatic, anti-inflammatory and antipyretic properties.

 

Key words: Bioactive compounds, chromatography-mass spectrometry (GC-MS) analysis, Mentha viridis