Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

Phenolic content, radical scavenging activity and cytotoxicity of Tamarix nilotica (Ehrenb.) bunge growing in Egypt

Riham Omar Bakr1*, Mohamed Abd El-Aziz El Raey2 and Rehab Seif Ashour3
1Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts, Giza, Egypt. 2Department of Phytochemistry and Plant Chemosystematics, National Research Center, Dokki, Cairo, Egypt. 3Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Egypt.
Email: [email protected], [email protected]

  •  Accepted: 28 January 2013
  •  Published: 31 March 2013

Abstract

The radical scavenging activity using 1,1-diphenyl, 2-picrylhydrazyl (DPPH) and cytotoxicity using sulphorhodamine B (SRB) assay of the aqueous methanolic extract ofTamarix nilotica (Ehrenb.) Bunge (Tamaricaceae) flowers and its subextracts (CHCl3, EtOAC and Pet.ether) were evaluated. Total phenolic and flavonoid contents were estimated using colorimetric assays. Ethyl acetate (EtOAc) showed the highest free radical scavenging activity with inhibitory concentration (IC50) 7.25 ± 0.86 µg/ml in addition to potential cytotoxic effect on liver cell carcinoma (Huh-7) (IC50 49.1 ± 0.96 µg/ml) whereas effect on lung cell carcinoma (A-549) was much lower (IC50: 137.9 ± 1.85 µg/ml). EtOAc had the highest flavonoid content (1.75 ± 1.5 mg/g QE) compared to other subextracts. These results indicated that ethyl acetate fraction contains bioactive compounds worthy of more sophisticated studies as free radical scavenger and cytotoxic agent.

 

Key words: Tamarix nilotica, cytotoxic activity, sulphorhodamine B (SRB) assay, scavenging activity, 1,1-diphenyl, 2-picrylhydrazyl (DPPH) assay, flavonoid, phenolic.