Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 228

Full Length Research Paper

Antitumoral effect of Striga hermonthica (Delile) Benth. methanolic extract is mediated by alterations on procaspase-3 and cyclin B expression in prostate cancer cell lines

Mindiédiba Jean BANGOU
  • Mindiédiba Jean BANGOU
  • Department of Biochemistry and Microbiology, Training and Research Unit in Sciences and Technology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.
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Tangbadioa Hervé COULIDIATI
  • Tangbadioa Hervé COULIDIATI
  • Laboratory of Biochemistry and Applied Chemistry, University of Ouaga I, Professor Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • Google Scholar
Aminata P NACOULMA
  • Aminata P NACOULMA
  • Laboratory of Biochemistry and Applied Chemistry, University of Ouaga I, Professor Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • Google Scholar
Abdoulaye SEGDA
  • Abdoulaye SEGDA
  • Department of Biochemistry and Microbiology, Training and Research Unit in Sciences and Technology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.
  • Google Scholar
Emmanuel AM THIOMBIANO
  • Emmanuel AM THIOMBIANO
  • Laboratory of Biochemistry and Applied Chemistry, University of Ouaga I, Professor Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso.
  • Google Scholar
Georges Anicet OUEDRAOGO
  • Georges Anicet OUEDRAOGO
  • Department of Biochemistry and Microbiology, Training and Research Unit in Sciences and Technology, University of Nazi BONI, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso.
  • Google Scholar


  •  Received: 21 January 2022
  •  Accepted: 16 March 2022
  •  Published: 30 April 2022

Abstract

Cancer is one of the major public health problems of the 21st century. Its incidence is increasing in both developed and low-income countries. This trend is justified by the change in people's lifestyles. Cancer of the prostate is one of major cancer types affecting male population. The present study focused on the use of methanolic extract of Striga hermonthica (Delile) Benth. to evaluate total phenolics and flavonoids contents, antioxidant activities, anti-proliferative and anti-migratory effects, using ATCC prostate cancer cell lines (22RV1, DU145, C4-2 and RWPE-1). Total phenolics and flavonoids content were estimated using spectrophotometric methods. Antioxidant potential was evaluated using three methods while cytotoxicity assay, anti-migratory assay and western blot analysis were used for the determination of extracts anti-proliferative activity on prostate cancer cells. Therefore, to assess apoptosis induction in prostate cancer cell lines, the effect of S. hermonthica (Delile) Benth. methanolic extract was investigated on procaspase-3, procaspase-8, and cyclin-B2 expression. A range of half-dilution concentrations beginning from 500 µg/mL have been prepared to determine the IC50 in the various tests. Antioxidant compounds were shown to be promising biomolecules, with particular interest in cancer management. Among plant derivative compounds, phenolics and derived molecules have endorsed antioxidant capacities and potential anti-proliferative activities. The best inhibition was obtained on C4-2 cells with an IC50 = 65.76 µg/mL and a selectivity index of 2.35. The results obtained from this study could partially justify the traditional indications of this plant in the treatment of prostate cancer.

Key words: Plant derived products, anti-tumoral, prostate cancer, antioxidants.