Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

New pentacyclic triterpene ester and flavone glycoside from the biologically active extract of Carduus pycnocephalus L.

Wafaa H. B. Hassan*
  • Wafaa H. B. Hassan*
  • Pharmacognosy Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
  • Google Scholar
Hanan M. Al-Youssef
  • Hanan M. Al-Youssef
  • Pharmacognosy Department, Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
  • Google Scholar
Latifa AL-Shammari
  • Latifa AL-Shammari
  • Pharmacognosy Department, Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
  • Google Scholar
Rehab H. Abdallah
  • Rehab H. Abdallah
  • Pharmacognosy Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
  • Google Scholar


  •  Received: 19 January 2015
  •  Accepted: 19 March 2015
  •  Published: 30 April 2015

Abstract

Bioassay guided phytochemical investigation of petroleum ether, chloroform and butanol  fractions obtained from biologically active total alcoholic extract of Carduus pycnocephalus led to isolation of  two new compounds, 3-O-acetyl-ursolic acid-28-ethyl ester (compound 1) and diosmetin-7-O-α-L-arabinopyransyl (1```→4``)-β-D- glucopyranoside (compound 5) along with  three  known compounds, bis (2-ethylhexyl) benzene-1,2-dicarboxylate) (compound 2),  3α, 24 dihydroxyolean-12-en-28, 30-dioic acid dimethyl ester (compound 3) and kaempferol (compound 4). Their structures were established on the basis of spectroscopic methods (UV, MS, 1H NMR, 13C NMR, COSY, HSQC and HMBC) and by comparison with published data. Compounds 2 and 3 were isolated for the first time from genus Carduus. The cytotoxic, antioxidant, and antimicrobial activities of the ethanolic extract were evaluated and the extract showed variable degrees of activities. It showed significant cytotoxic activity against MCF-7, A-549 and HepG-2 cell lines with IC50, 17.9, 17.5  and  21.8 µg, respectively. The extract displayed weak antioxidant activity by scavenging of DPPH with SC50 554.2 µg. In agar diffusion assay, the extract exhibited strong antibacterial activities against Gram negative bacteria Pseudomonas aeruginosa and Escherichia coli and strong antifungal activity against Syncephalastrum racemosum.

Key words: Carduus pycnocephalus L.; new triterpene ester, flavonoidal glycoside, antioxidant, cytotoxic, antimicrobial activity.