Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

Gas chromatography mass spectrum and fourier-transform infrared spectroscopy analysis of methanolic extract of Rosmarinus oficinalis leaves

Imad Hadi Hameed*
  • Imad Hadi Hameed*
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Israa Adnan Ibraheam
  • Israa Adnan Ibraheam
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Hawraa Jawad Kadhim
  • Hawraa Jawad Kadhim
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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  •  Received: 20 April 2015
  •  Accepted: 03 June 2015
  •  Published: 30 June 2015

Abstract

The aims of the study were to investigate the presence of phytochemical compounds from the leaves of Rosmarinus oficinalis, using methanolic extraction and report the main functional components by using FT-IR. The phytochemical compound screened by GC-MS method. Seventeen bioactive phytochemical compounds were identified in the methanolic extract of R. oficinalis. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, chemical structure, MS Fragment ions and pharmacological actions. GC-MS analysis of Rosmarinus oficinalis revealed the existence of the α-pinene, Camphene, Eucalyptol, 2-Methoxy-4-vinylohenol, 1-Oxaspiro[4,5]deca-3,6-diene,2,6,10,10-tetramethyl, 1-Oxaspiro[4,5] deca-3,6-diene,2,6,10,10-tetramethyl, Neocurdione, Isoaromadendrene epoxide, 1b,4a-Epoxy-2H-cyclopenta[3,4]cyclopropa[8,9] cycloundec., Cis-Vaccenic acid, Phenanthrenol,4b,5,6,7,8,8a,9,10-octahydro-4b,8,8-trimethyl-1, Galanthamine, Dibenz[a,c]cyclohexane,2,4,7-trimethoxy, 2,4a,7-Trihydroxy-1-methyl-8-methyleneqibb-3-ene. 1,10-carboxylic acid, Retinoic acid, 7,8,12-Tri-O-acetyl-3-desoxy-ingol-3-one and 4,6-Androstadien-3β-ol-17-one,acetate. The FTIR analysis of Rosmarinus oficinalis leaves proved the presence of Alkenes, Aliphatic fluoro compounds, Alcohols, Ethers, Carboxlic acids, Esters, Nitro compounds, Alkanes, Aldehydes, Ketones compounds. It contain chemical constitutions which may be useful for various herbal formulation as anti-inflammatory, analgesic, antipyretic, cardiac tonic and antiasthamatic.  

 

Key words:  FT-IR, GC-MS analysis, Leaves, Methanol, Rosmarinus oficinalis.