Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

Antimicrobial, antioxidant and in vitro anti-inflammatory activity of ethanol extract and active phytochemical screening of Wedelia trilobata (L.) Hitchc.

Govindappa M., Naga Sravya S., Poojashri M. N., Sadananda T. S. and Chandrappa C.P.
Department of Biotechnology, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur-572 106,   Karnataka, India. 
Email: [email protected]

  •  Accepted: 28 April 2011
  •  Published: 30 April 2011

Abstract

Ethanol extract of leaf, stem and flower of Wedelia trilobata was assessed for its antimicrobial, 
antioxidant and anti-inflammatory activity and phytochemical screening. Total phenolic content was 
assessed using Folin-Ciocalteu’s method. The antioxidant activity was determined by measuring the 
scavenging activity of DPPH radical and FRAP assay. The antimicrobial efficacy was determined using 
paper disc method against different fungi and bacteria. Sensitivity in terms of zones of inhibition and
phytochemical composition of the all parts extracts were also determined. In vitro anti-inflammatory 
activity was evaluated using albumin denaturation, membrane stabilization assay and proteinase 
inhibitory assay. Aspirin was used as a standard drug for the study of anti-inflammatory activity. The
results show that, all parts extracts effective against all the bacteria tested, whereas all the extracts were 
failure in inhibiting the growth of all Alternaria sp., Cercospora carthami and Nigrospora oryzae, but 
other fungi also were showed moderate inhibition against all the three extracts. Phytochemical analysis 
revealed the presence of tannins, flavonoids, terpenoids, phenols and saponins. Leaf and stem ethanol 
fractions showed highest total Phenolic content. The leaf and stem ethanol extract possessed strong 
scavenging activity in both DPPH and FRAP methods. In DPPH and FRAP method, the leaf and stem had 
showed free radical inhibition of 86, 82 and 630.72, 508.81 respectively. The leaf and stem ethanol extract 
also showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation and 
red blood cells membrane stabilization with 87.14 and 86.76 and 78.11, 74.17 g/ml respectively. 
Proteinase activity was also significantly inhibited by the leaf (84.19 g/ml) and stem (81.84 g/ml). From 
the result, it is concluded that phytochemicals (tannins, flavonoids, terpenoids, phenols and saponins) 
present in the W. trilobata extract may be responsible for the antimicrobial, antioxidant and antiinflammatory activity. 
 
 
Key words: Wedelia trilobata, antimicrobial, antioxidant, anti-inflammatory, phytochemicals.