Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 195

Full Length Research Paper

Clinopodium nubigenum (Kunth) Kuntze essential oil: Chemical composition, antioxidant activity, and antimicrobial test against respiratory pathogens

Paco Fernando Noriega
  • Paco Fernando Noriega
  • Research and Development Group in Sciences Applied to Biological Resources (GIDCARB), Salesian Polytechnic University, Quito-Ecuador.
  • Google Scholar
Tatiana de Los Ángeles Mosquera
  • Tatiana de Los Ángeles Mosquera
  • Research and Development Group in Sciences Applied to Biological Resources (GIDCARB), Salesian Polytechnic University, Quito-Ecuador.
  • Google Scholar
Edison Antonio Osorio
  • Edison Antonio Osorio
  • Research and Development Group in Sciences Applied to Biological Resources (GIDCARB), Salesian Polytechnic University, Quito-Ecuador.
  • Google Scholar
Pablo Guerra
  • Pablo Guerra
  • Research and Development Group in Sciences Applied to Biological Resources (GIDCARB), Salesian Polytechnic University, Quito-Ecuador.
  • Google Scholar
Andrea Fonseca
  • Andrea Fonseca
  • Research and Development Group in Sciences Applied to Biological Resources (GIDCARB), Salesian Polytechnic University, Quito-Ecuador.
  • Google Scholar


  •  Received: 02 August 2017
  •  Accepted: 25 June 2018
  •  Published: 30 September 2018

Abstract

The essential oil of leaves and flowers of Clinopodium nubigenum (Kunth.) Kuntze (Lamiaceae) collected in the province of Pichincha-Ecuador was steam distilled and analyzed by gas chromatography mass spectrometry (GC/MS) to determine its chemical composition. The majority of the compounds identified were carvacrol acetate (42.1%), carvacrol (20.6%), pulegone (6.3%) and thymol (5.5%). Antioxidant activity was assessed by the assays of diphenylpicrylhydrazyl (DPPH) (IC50: 1.8 μl / ml), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) (IC50: 0.3 μl / ml) and β-carotene (IC50: 0.031 μl / ml) compared to Thymus vulgaris and butylated hydroxyanisole (BHA) as referents. The specie also shows a promising medicinal potential exhibiting significant antibacterial activity at different concentrations against Staphylococcus aureus (2.5% v/v), Streptococcus pyogenes (0.6% v/v), Streptococcus pneumoniae (0.6% v/v) and Streptococcus mutans (0.6% v/v), suggesting an interesting natural alternative in the fight against bacteria that generate resistance to other antibiotics.

Key words: Clinopodium nubigenum, gas chromatography mass spectrometry (GC/MS), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), diphenylpicrylhydrazyl (DPPH), antimicrobial test.