Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

Bioactivity-directed separation of an anxiolytic fraction from Aethusa cynapium L.

Richa Shri1*, Manjeet Singh1 and Anupam Sharma2
1Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India. 2Pharmacognosy Division, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
Email: [email protected]

  •  Accepted: 16 July 2009
  •  Published: 31 March 2010

Abstract

The present study has been designed to evaluate the putative anxiolytic activity of petroleum ether, chloroform, methanol and water extracts of the aerial parts ofAethusa cynapium using the widely accepted elevated plus-maze model in mice. An attempt has been made to isolate the bioactive fraction by resorting to bioactivity directed fractionation and chromatographic procedures like column and flash chromatography. Fraction 3.1.3.2, which was derived from the methanol extract of the plant, showed significant anxiolytic activity at a dose of 50 mg/kg as compared to the standard drug, diazepam (2 mg/kg p.o.). This subfraction comprises of two flavonoid components. The study validates the traditional use of A. cynapium for the treatment of anxiety.

 

Key words: Aethusa cynapium L., anxiolytic effect, elevated plus-maze model, column and flash chromatography.