Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 193

Full Length Research Paper

Protective effect of ethanolic extract of Cucurbita maxima (PUMPKIN) leaf on acetaminophen-induced acute liver toxicity

Michael Okey Enemali
  • Michael Okey Enemali
  • Department of Biochemistry and Molecular Biology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi, Nasarawa State, Nigeria.
  • Google Scholar
Titilayo Oluwayemisi Bamidele
  • Titilayo Oluwayemisi Bamidele
  • Department of Biochemistry and Molecular Biology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi, Nasarawa State, Nigeria.
  • Google Scholar
Mohammed Abdullahi Ubana
  • Mohammed Abdullahi Ubana
  • Department of Biochemistry and Molecular Biology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi, Nasarawa State, Nigeria.
  • Google Scholar

  •  Received: 15 March 2018
  •  Accepted: 18 June 2018
  •  Published: 31 August 2018


Afolayan M, Salisu A, Adebiyi A, Idowu D, Fagbohun A (2014). In vitro antioxidant,antimicrobial and phytochemical properties of wild banana [Ensete illetii (E. A. J. DE Wildman)] seeds extract. International Journal of Advanced Chemistry 2(2):59-61.


Aja PM, Okaka ANC, Onu PN, Ibiam U, Urako AJ (2010). Phytochemical Composition of Talinum triangulare (Water Leaf) Leaves. Pakistan Journal of Nutrition 9(6):527-530.


Alamgir HM, Sheikh AM, Muniruddin A, Shahidulla KMD (2016). Phytochemical and Pharmacological Investigation of Lagenaria siceraria, Cucumis sativus and Cucurbita maxima. European Journal of Medicinal Plants 12(3):1-13


Al-Mamary M, Al-Habori M, Al-aghbari AM, Basker MM (2002). Investigation into the toxicological effects of Catha edulis leaves. A short term study in animals. Phytotherapy Research 16(2):127-132.


Association of Analytical Chemist (2006). Official methods of analysis, 21st edition. Association of Analytical Chemist, Washington D.C. pp. 74-175 .


Bancroft JD, Suvarna KS, Christopher L (2013). Bancroft's Theory and Practice of Histological Techniques. Seventh ed. Churchill Livingstone-Elsevier.


Beers RF,Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J. Biol.Chem. 195: 133-140.Chakravarthy HM (1982). Fascicles of flora of India – 11 Cucurbitaceae. Botanical Survey of India 136 p.


Dahanukar A, Foster K, Van DG, Van NWM, Carlson JR (2001). A G- receptor is required for response to the sugar trehalose in taste neurons of Droshila. Nature Neuroscience 4:1182-1186.


Dahlin DC, Miwa GT, Lu AYH, Nelson SD (1984). N- Acetyl-p-benzoquinone imine: A cytochrome P-450-mediated oxidation product of acetaminophen. Proceedings of the National Academy of Sciences of the United States of America 81:1327-1331.


Ekor M, Adepoju GKA, Epoyun AA (2006). Protective Effect of Methanolic Leaf extracts of Persea americana (Avocado) Against Paracetamol-Induced Acute Hepatotoxicity in Rats. International Journal of Pharmacology 2(4):416-420.


Erica D, Emily MA (2014). Acetaminophen Toxicity: What Pharmacists Need to Know? US Pharmacist. 39(3):HS2-HS8


Ezekwe CI, Uzomba CR, Ugwu OPC (2013).The Effect of Methanol Extract of Talinum Triangulare (Water Leaf) on the Hematology and Some Liver Parameters of Experimental Rats.Global Journal of Biotechnology and Biochemistry 8(2):51-60.


Jack AH, Dean WR, Laura PJ (2009). Mechanisms of Acetaminophen-Induced Liver Necrosis Handbook of Experimental Pharmacology 196:369-405.


Jain N, Pathak A (2012). Hepatoprotective Effect of Methanolic Extract of C. maxima and L. siceraria Seeds. International Journal of Pharmaceutical, Chemical and Biological Science 2(2):151-154.


James LP, Letzig L, Simpson PM, Capparelli E, Roberts DW, Hinson JA, Lee WM (2009). Pharmacokinetics of acetaminophen-protein adducts in adults with acetaminophen overdose and acute liver failure. Drug Metabolism and Disposition 37:1779-1784.


King EJ (1965a). The hydrolases-acid and alkaline phosphatases. In Van D (ed.): Practical Clinical Enzymology, Nostrand Co., London pp. 199-208.


King EJ (1965b).The transaminases: alanine and aspartate transaminases. In Van D (ed.): Pract Clin Enzy, Nostrand Co., London pp. 363-395.


Ladipo MK, Doherty VF, Kanife UC (2010). Phytochemical Screening and Antibacterial Investigation of the Extract of Ocimum Gratissimum (Scent Leaf) on Selected Enterobacteriaceae.


Lindhorst TK (2007). Essentials of Carbohydrate Chemistry and Biochemistry. Wiley-VCH. ISBN 978-3527315284.


Lawrence R, Burk R (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and Biophysical Research Communications 71:952-958.


Lowry OH, Rosebrough NJ, Farr AL, Randal RJ (1951). Protein measurement with Follin's Phenol reagent. Journal of Biological Chemistry 193:265-275.


Malloy HT Evlyn EA (1937). The determination of bilirubin with photoelectric calorimeter. Journal of Biological Chemistry 119:481-485.


Martin P, Friedman LS (1992). Assessment of liver function on Diagnostic studies In: Friedman L. S. and Keefe, E. B (Eds). Handbook of liver disease. Churchil Livingstone, Philadelphia pp. 1-14.


Mitchell JR, Jollow DJ, Potter WZ, Gillette JR, Brodie BB (1973). Acetaminophen-induced hepatic necrosis iv, Protective role of glutathione. Journal of Pharmacology and Experimental Therapeutics 187:211-217.


Mayuresh R, Andrzej P, Patrycja LK, Wojciech Z, Rafal F (2014) Herbal medicine for treatment and prevention of liver diseases. J.Pre-clin and clin. 8(2):55-60.


Okediran BS, Olurotimi AE, Rahman SA, Michael OG Olukunle JO (2014). Alterations in the lipid profile and liver enzymes of rats treated with monosodium glutamate. Sokoto Journal of Veterinary Sciences 12(3):42-46.


Olamide EA, Mathew OA (2013). Protective Effects of Enantia chlorantha Stem Bark Extractson Acetaminophen Induced Liver Damage in Rats. Jordan Journal of Biological Sciences 6(4):284-290.


Olorunnisola OS, Bradley G, Afolayan AJ (2011). Antioxidant properties and Cytotoxicity evaluation of methanolic extract of dried and fresh rhizomes of Tulbaghia violacea. African Journal of Pharmacy and Pharmacology 5:2390-2497.


Perez Gutierrez RM (2016). Review of Cucurbita pepo (Pumpkin), its Phytochemistry and Pharmacology. Perez Gutierrez, Medicinal Chemistry 6:012-021.


Prabu K Kanchana N, Mohammed SA (2011). Hepatoprotective effect of Eclipta alba on paracetamol induced liver toxicity in rats. Journal of Microbiology and Biotechnology Research 1(3):75-79)


Prerona SUK, Mazumder PK, Haldar AB, Biswakanth K, Sagar N (2011). Evaluation of Hepatoprotective Activity of Cucurbita Maxima Aerial Parts. Journal of Herbal Medicine and Toxicology 5(1):17-22


Reed S (2009). Essentials of physiological Biochemistry; an organ based approach. John Willey and sons ltd West Sussex, Uk


Sarvesh DD (2012). Overview on Cucurbita maxima.International Journal of Phytopharmacy 2(3):68-71.


Shahlah JA Al-Shaheen, Raad A Kaskoos, Khitam JH, Javed A (2013) In-vitro antioxidant and α-amylase inhibition activity of Cucurbita maxima. Journal of Pharmacognosy and Phytochemistry 2 (2):121-124.


Sun M Zigma S (1978). An improved spectrophotometric assay of superoxide dismutase based on ephinephrine antioxidation. Analalytic Biochemistry 90:81-89.


Udochukwu U, Omeje FI, Uloma I S, Oseiwe FD (2015). Phytochemical analysis of Vernonia amygdalina and Ocimum gratissimum extracts and their antibacterial activity on some drug resistant bacteria. American Journal of Research Communication 3(5):225-235


Usoh FI, Akpan EJ, Etim EO Farombi EO (2005). Antioxidant actions of dried flower of Hibiscus sabidariffa L. on sodium arsenite- induced oxidative stress. Pakistan Journal of Nutrition 4:135-141.


Vidhya M, Bai M (2012). Beware of Paracetamol Toxicity. Journal of Clinical Toxicology pp. 1-3.